B16-F0 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300308

安全資料表

產品說明書

分析證明書 (CoA)

說明	The B16-F0 cell line is a murine melanoma cell line derived from the B16 mouse melanoma. This cell line is widely used in cancer research due to its high metastatic potential and ability to form tumors when injected into syngeneic mice. B16-F0 cells are particularly useful for studying the molecular mechanisms underlying melanoma progression and metastasis, as well as for testing the efficacy of anti-cancer drugs and therapeutic interventions in preclinical models. Notably, the B16-F0 cell line is the parent cell line from which other variants, such as B16-F1, B16-F10, and B16-BL6, have been derived through selective procedures aimed at enhancing specific metastatic properties. B16-F0 cells exhibit a typical epithelial morphology and grow adherently in culture. They are known to express various melanoma-associated antigens, making them a valuable tool for immunological studies and for developing melanoma vaccines. Additionally, these cells are often employed in studies involving gene expression, signaling pathways, and the tumor microenvironment. Researchers utilize B16-F0 cells to explore the interactions between melanoma cells and the immune system, particularly focusing on immune evasion and suppression mechanisms. The characterization of B16-F0 and its derived lines provides a comprehensive framework for understanding the invasive and metastatic behaviors of melanoma, with B16-F1, B16-F10, and B16-BL6 each representing stages of increasing metastatic and invasive activity, thereby serving as critical models in the study of cancer progression and therapeutic response.
生物體	Mouse
組織	Skin
疾病	Mouse melanoma
同義詞	B16/F0, B16F0

品種／亞種	C57BL/6
性別	Male
形態學	Mixture of spindle-shaped and epithelial-like cells
細胞類型	Epithelial
生長特性	Adherent

引用	B16-F0 (Cytion catalog number 300308)
生物安全等級	1
NCBI_TaxID	10090
Cellosaurus 編號	CVCL_0604

致瘤性	Yes, in syngeneic mice
產品	Melanin

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300308-250324	分析證明書	23. May. 2025	300308

B16-F0 Cells

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品質控制與分子分析

分析證明書 (CoA)