AML12 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300643

安全資料表

產品說明書

分析證明書 (CoA)

說明	AML12 cells, also known as Alpha Mouse Liver 12 cells, are a non-tumorigenic epithelial cell line derived from the liver of a transgenic mouse. These cells were initially developed to provide a suitable in vitro model for studying the hepatocyte function and liver biology of the adult mouse. AML12 cells express characteristics typical of differentiated hepatocytes, including the production of albumin, transferrin, and other liver-specific proteins, making them an invaluable resource for research in toxicology, drug metabolism, and liver disease. The cell line was established from hepatocytes isolated from a mouse harboring a transgene for human transforming growth factor alpha (TGF-alpha), under the control of the mouse metallothionein-I promoter. This genetic alteration contributes to the immortalization of the cells without disrupting their differentiated state. AML12 cells maintain a stable phenotype and karyotype under standard cell culture conditions, which includes a unique requirement for dexamethasone and insulin-transferrin-selenium in the growth medium to promote proliferation and maintain hepatocyte-specific functions.
生物體	Mouse
組織	Liver
應用	3D cell culture, High-throughput screening, Toxicology
同義詞	AML-12, AML 12, Alpha Mouse Liver 12

品種／亞種	CD-1 MT42 transgenic
年齡	3 months
性別	Male
形態學	Epithelial
細胞類型	Hepatocyte
生長特性	Adherent

引用	AML12 (Cytion catalog number 300643)
生物安全等級	1
NCBI_TaxID	10090
Cellosaurus 編號	CVCL_0140
轉基因狀態	GMO-S1: This murine hepatocyte cell line (AML12) contains a human TGF-α transgene introduced by transfection, enabling growth-factor-dependent signaling studies. The insert is stably integrated into hepatocytic cells. This classification applies only within Germany and may differ elsewhere.

產品	The cells express high levels of human TGF alpha and lower levels of mouse TGF alpha.

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 10% FBS, 10 microgram/mL insulin, 5.5 microgram/mL transferrin, 5 ng/mL selenium, 40 ng/mL dexamethasone
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Info: Dexamethasone to be added freshly to culture medium

Stability in medium limited to ~3–5 days under standard cell culture conditions

Prolonged incubation (37 °C, 5 % CO₂) may result in gradual degradation

批次編號	證書類型	日期	型號
300643-280525	分析證明書	21. Jul. 2025	300643
300643-301123	分析證明書	23. May. 2025	300643
300643-160226	分析證明書	13. Mar. 2026	300643

AML12 Cells

一般資訊

特徵

監管數據

生物分子資料

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品質控制與分子分析

分析證明書 (CoA)