V79 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€420.00*

Prices excl. taxes plus shipping costs

Product number: 305012

Safety data sheet

Product sheet

Description	V79 cells are a Chinese hamster lung fibroblast cell line, commonly used in genetic, toxicological, and radiobiological research. They originate from the lung tissue of the Chinese hamster and are particularly valued for their rapid growth rate and stable karyotype, making them a reliable model for various laboratory studies. One of the primary uses of V79 cells is in cytotoxicity and genotoxicity testing. These cells are employed to assess the potential DNA-damaging effects of chemical compounds and radiation, providing crucial data for risk assessment and safety evaluations. V79 cells are highly responsive to mutagens and carcinogens, making them an excellent choice for mutagenicity assays, such as the micronucleus test and chromosome aberration test. In radiation biology, V79 cells are used to study the effects of ionizing radiation on cellular structures and to evaluate the efficacy of radioprotective substances. Their sensitivity to radiation-induced damage allows researchers to investigate the mechanisms of DNA repair, cell cycle arrest, and apoptosis following exposure to various types of radiation. V79 cells are also instrumental in pharmacological research, particularly in drug screening processes where their robust growth and reproducibility are advantageous for high-throughput assays. They are used to test the cytotoxic effects of new drugs and to study the cellular uptake and metabolism of pharmaceutical compounds. Overall, the V79 cell line is a versatile tool in biomedical research, contributing to our understanding of cellular responses to environmental agents and aiding in the development of safer and more effective therapeutic interventions.
Organism	Hamster
Tissue	Lung
Applications	V79 cells are a widely used and established cell line in biological research, particularly in the study of DNA repair and DNA damage. These cells have a shortened cell cycle, are readily mutagenized to make stable mutant lines deficient in DNA repair enzymes and related DNA damage response functions, and are particularly useful for gene toxicity assays due to their stability of karyotype and morphology. V79 cells have been widely utilized in studies on x-ray, UV radiation, and oxidizing agent-induced DNA damage and repair, as well as investigations into cellular signaling pathways, apoptosis, inflammation, and the effects of various chemicals and compounds on cellular growth and viability. Their extensive use in research attests to their usefulness and importance in biological science.
Synonyms	V-79, V 79, Strain V, V79-1, GM00215, GM-215, GM00215A, GM16136, UCW 100

Gender	Male
Morphology	Fibroblast
Growth properties	Adherent

Citation	V79 (Cytion catalog number 305012)
Biosafety level	1

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (Cytion catalog number 800100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

V79 Cells

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