NCI-H460 Cells - Navigating Lung Cancer Research with NCI-H460 Insights
NCI-H460 are human-derived non-small cell lung cancer cells commonly used in lung carcinoma and toxicology research. This cell line is a valuable tool for studying various cancer biology aspects involving tumour development, growth, and drug resistance. Moreover, NCI-H460 cells are an appropriate model for developing anti-cancer reagents.
NCI-H460 cells: Origin and general characteristics
The origin and general characteristics of a cell line majorly contribute to its research applications. This section of the article will assist you in learning the origin and salient features of NCI-H460 lung cancer cells. You will know: What are NCI-H460 cells? What is the NCI-H460 cell line type? What is the morphology of NCI-H460?
- The NCI-H460 cell line originated from the pleural effusion of a European male with large-cell lung cancer. It was established in 1982 by A.F. Gazdar and colleagues.
- NCI-H460 lung cancer cells possess an epithelial morphology.
- NCI-H460 is a tumorigenic cell line with a hypotriploid karyotype. The modal chromosome number for these cells is 57. NCI-H460 cells also possess 58 modal chromosome numbers at comparable rates.
- These lung cancer cells have many NCI-NCI-H460 mutations like non-small cell lung tumours, such as NCI-H460 KRAS mutation, which are involved in cell proliferation, growth, invasion, and metastasis.
NCI-H460 Cells Culturing Information
You should know the following key points for proper handling and maintenance of the NCI-H460 cell line. It will inform you about NCI-H460 doubling time, NCI-H460 culture medium, and basic cell culture procedures for NCI-H460 lung cancer cells.
Key Points for Culturing NCI-H460 Cells
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Doubling Time: |
The NCI-H460 doubling time is approximately 33 hours. |
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Adherent or in Suspension: |
The NCI-H460 lung cancer cells are adherent. |
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Sub-cultivation Ratio: |
The split ratio recommended for the NCI-H460 cell line is 1:2 and 1:4. After removing the old medium, adherent cells are rinsed with 1 x phosphate buffer saline. Afterwards, cells are incubated with Accutase passaging solution for 8 to 10 minutes at ambient temperature. Dissociated cells are resuspended in a culture medium and centrifuged. Harvested cells are again resuspended and poured into the new flask for culturing. |
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Growth Medium: |
RPMI 1640 is used as NCI-H460 culture medium. It is supplemented with 10% fetal bovine serum, 2.1 mM stable Glutamine, and 2.0 g/L NaHCO3. Media should be replaced 2 to 3 times a week. |
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Growth Conditions: |
NCI-H460 cultures are maintained at 37°C temperature in a humidified incubator with a continuous 5% CO2 supply. |
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Storage: |
NCI-H460 lung cancer cells can be stored in the vapour phase of liquid nitrogen or at below -150°C temperature in an electric ultra-low temperature freezer for the long term. |
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Freezing Process and Medium: |
CM-1 or CM-ACF medium is used to freeze and store NCI-H460 cells. A slow freezing method is recommended to protect maximum cell viability. |
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Thawing Process: |
Frozen NCI-H460 cells are thawed in a pre-warmed water bath (at 37°C temperature) for 40 to 60 seconds until a small ice clump is left. Thawed cells are added with fresh media and centrifuged to remove freezing media components. The collected cell pellet is again resuspended, and cells are dispensed into new flasks containing media for growth. NCI-H460 cells may take almost 24 hours to attach to the surface of the flask. |
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Biosafety Level: |
NCI-H460 lung cancer cells are handled and maintained in biosafety level 1 laboratories. |
Advantages & Disadvantages of NCI-H460 cells
NCI-H460 is a widely used cell line in lung cancer research. This section will cover the general advantages and disadvantages related to NCI-H460 lung cancer cells.
Advantages
The advantages of the NCI-H460 non-small cell lung cancer cell line are:
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Tumour origin
The NCI-H460 cell line was derived from a large-cell lung carcinoma patient, representing this particular lung cancer type. They are used as a model to study lung cancer biology and develop new and effective treatments. NCI-H460 cells have tumorigenic potential and can be injected in immunodeficient mice to create in vivo tumour models for studying tumour growth, development, and efficacy of potential drugs.
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High proliferation rates
NCI-H460 exhibits higher growth rates than other non-small cell lung cancer cell lines, such as A549. This advantage increases their availability and helps researchers conduct reproducible and time-sensitive experiments.
Disadvantages
The disadvantages associated with NCI-H460 lung cancer cells are:
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Homogeneity
NCI-H460 cells are homogenous as they were obtained from a single patient tumour. Therefore, they generally lack complexity and heterogeneity observed in patient tumours.
Research applications of NCI-H460 cells
The NCI-H460 lung cancer cells are extensively used in studies related to lung carcinoma. Here are some of the important research applications of NCI-H460 cells:
- Lung cancer research: NCI-H460 cells are an invaluable model for investigating cell and molecular mechanisms involved in tumour development, growth, and metastasis. Moreover, it is used to study imperative signalling pathways, molecular targets, and various genetic mutations associated with lung cancer progression. Several studies have been conducted on NCI-H460 cells to study these factors effectively. A study conducted in 2019 proposed that overexpressed nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) are involved in tumour cell growth via regulation of the PI3K/AKT signalling pathway [1]. Similarly, an in vitro and in vivo study employed NCI-H460 cells to study the role of the eIF4E gene. The findings revealed that the eIF4E gene is involved in lung carcinoma growth and angiogenesis, and it can be targeted to develop promising anti-lung cancer drugs [2].
- Drug discovery and development: NCI-H460, a human lung cancer cell line, is widely used in drug discovery and development studies. Researchers use these cells to investigate the toxicity and efficacy of novel drug candidates, targeted therapies, and treatments mainly targeting NCI-H460 KRAS mutations. A study conducted by Haoyue Hu and colleagues in 2023 employed NCI-H460 cells to study the anti-cancer effects of the anlotinib drug. The results showed that anlotinib partly affected the growth of KRAS mutant lung cancer cells by inhibiting the MEK/ERK signalling cascade [3]. Likewise, a phenolic compound, carnosic acid was screened for antiproliferative and proapoptotic activities using NCI-H460 cells [4].
- Drug resistance: The NCI-H460 cell line is ideal for studying lung carcinoma's drug resistance mechanism. Researchers use these cells to develop drug resistance models to identify underlying genes, molecular factors, and signalling pathways. Such as, a study developed Pemetrexed, a multitargeted antifolate agent, resistant NCI-H460 cells to study underlying molecular mechanisms of pemetrexed resistance in non-small cell lung cancer cells [5].
Purchase the NCI-H460 Cell Line: Your Gateway to Lung Cancer Research
Research Publications Featuring NCI-H460 Cells
Here are some interesting research publications about the NCI-H460 lung carcinoma cell line.
A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway
This study in the Molecules (2023) proposed that a natural α-1,6-glucan, BBWPW, from black bean inhibits the proliferation of NCI-H460 cells via regulating PI3K/AKT/MAPK pathway.
This article in Phytomedicine (2019) studied that dioscin-6′-O-acetate, a novel natural compound, exerts an antiproliferative effect on NCI-H460 lung cancer cells.
miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis
BMC Pulmonary Medicine (2020) research states that microRNA-425-5p enhances lung carcinoma tumorigenesis through PTEN/PI3K/AKT pathway.
This article in Molecular Medicine Reports (2017) proposed the antitumor potential and underlying mechanisms of the quinalizarin compound in NCI-H460 and other lung cancer cells.
This research in the Food and Function (2019) highlights the potential anti-cancer effect of Eucalyptus globulus labill. extract using NCI-H460 cells. The findings showed that plant extract showed these effects by increasing NCI-H460 p53 expression and modulating the cell cycle profile.
Resources for NCI-H460 Cell line: Protocols, Videos, and More
Here are some online resources featuring the NCI-H460 lung cancer cells.
- NCI-H460 cell transfection: This video tutorial is a step-by-step guide for transfecting NCI-H460 cells with plasmid DNA.
The following links contain imperative cell culture information for H460 cells.
- NCI-H460 cells: This website will provide important information about NCI-H460 cell media, subculturing, freezing, and thawing procedures.
- Passaging NCI-H460 cells: This document will guide you about the passaging and subculturing of the NCI-H460 cell line. Besides, it will also help you learn transfection protocol for NCI-H460 cells.
Exploring the NCI-H460 Cell Line: FAQs and Insights
The NCI-H460 cell line originates from a patient with large cell lung carcinoma, making it a valuable model for studying this type of lung cancer.
NCI-H460 cells are utilized to investigate lung cancer biology, develop new treatments, and assess the efficacy of potential drugs. They can also be injected into immunodeficient mice to create in vivo tumor models for studying tumor growth and development.
Both NCI-H460 and A549 cell lines are derived from lung carcinomas, but NCI-H460 represents large cell carcinoma, while A549 represents adenocarcinoma. This difference in histological subtype influences their characteristics and behavior.
Yes, NCI-H460 cells have tumorigenic potential and can be implanted into immunodeficient mice to create xenograft models, facilitating the study of large cell lung carcinoma in vivo.
Combining paclitaxel with other compounds may enhance its cytotoxic effects on NCI-H460 cells, potentially improving treatment outcomes in lung cancer.
Yes, NCI-H460 cells express hypoxanthine guanine phosphoribosyltransferase, which is essential for nucleotide metabolism and cell proliferation.
NCI-H460 cells are commonly used in cancer and toxicology research to study tumor growth, assess cytotoxic effects of compounds, and investigate mechanisms of drug resistance.
NCI-H460 cells typically express neurofilament triplet proteins, which are involved in maintaining cytoskeletal structure and may play a role in cancer cell migration and invasion.
The κB pathway, among others, is implicated in regulating NCI-H460 cell proliferation and survival, offering potential targets for therapeutic intervention in lung cancer.
Yes, NCI-H460 cells are commonly used in vitro models to assess the cytotoxic effects of chemotherapeutic agents, including paclitaxel and carboplatin, aiding in the development of effective cancer treatments.
References
- Hu, C., et al., NUCKS Promotes the Proliferation, Migration and Invasion of Lung Cancer Cells Through Pi3k/Akt Signalling Pathway. Clinical and Investigative Medicine, 2021. 44(2): p. E55-61.
- Qi, X., et al., EGPI-1, a novel eIF4E/eIF4G interaction inhibitor, inhibits lung cancer cell growth and angiogenesis through Ras/MNK/ERK/eIF4E signaling pathway. Chemico-Biological Interactions, 2022. 352: p. 109773.
- Hu, H., et al., Anlotinib exerts anti-cancer effects on KRAS-mutated lung cancer cell through suppressing the MEK/ERK pathway. Cancer Management and Research, 2020: p. 3579-3587.
- Corveloni, A.C., et al., Carnosic acid exhibits antiproliferative and proapoptotic effects in tumoral NCI-H460 and nontumoral IMR-90 lung cells. Journal of Toxicology and Environmental Health, Part A, 2020. 83(10): p. 412-421.
- Xu, Y.-L., et al., Establishment and characterization of pemetrexed-resistant NCI-H460/PMT cells. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 2019. 19(6): p. 731-739.