Human Dental Follicle stem Cells (hDFSC) Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€800.00*

Content: 1 cryovial

Prices excl. taxes plus shipping costs

Product number: 300701

Safety data sheet

Product sheet

Description	Human Dental Follicle Stem Cells (DFSCs, hDFSCs) are a type of mesenchymal stem cell (MSC) derived from the dental follicle, an ectomesenchymal tissue surrounding the developing tooth germ. These cells are of particular interest in regenerative medicine due to their multipotent capabilities, meaning they can differentiate into various cell types, including osteoblasts (bone-forming cells), chondrocytes (cartilage-forming cells), adipocytes (fat cells), and possibly neural cells. DFSCs are typically harvested from the dental follicles of impacted third molars (wisdom teeth) and are valued for their ease of accessibility and minimal ethical concerns compared to other stem cell sources. DFSCs exhibit several key properties that make them promising for therapeutic applications. They possess strong proliferative abilities, maintaining their capacity to self-renew over extended culture periods. Moreover, they have a notable ability to migrate and home to injury sites, a characteristic that enhances their potential for use in tissue engineering and repair. DFSCs also secrete a range of bioactive factors that contribute to their immunomodulatory effects, making them valuable in the treatment of inflammatory conditions. Research into DFSCs has shown their potential in dental tissue engineering, particularly in the regeneration of periodontal tissues, pulp, and bone. Additionally, their differentiation into neural-like cells opens avenues for neurological applications. Despite the promising attributes of DFSCs, further studies are required to fully understand their differentiation pathways, optimize culture conditions, and confirm their long-term safety and efficacy in clinical settings.
Organism	Human
Tissue	Dental

Growth properties	Adherent

Citation	Human Dental Follicle stem cells (DFSC, hDFSC) (Cytion catalog number 300701)
Biosafety level	1

Culture Medium	Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3
Medium supplements	Supplement the medium with 10% FBS, 2 ng/mL bFGF
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	2 x 10^4 cells/cm^2
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Human Dental Follicle stem Cells (hDFSC) Cells

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA