Human Mesenchymal Stem Cells - Bone Marrow (HMSC-BM)

€690.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 5 × 10⁵ cells for primary cell lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300665

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	Human Mesenchymal Stem Cells derived from Bone Marrow (HMSC-BM) represent a robust and versatile tool for in vitro research. These multipotent mesenchymal stromal cells (MSCs) possess the unique ability to self-renew and differentiate into a broad spectrum of cell types, including adipocytes, osteoblasts, and chondrocytes. The potential of HMSC-BM to differentiate into these three key lineages has been well-documented, making them invaluable for studies focused on regenerative medicine, tissue engineering, and cellular differentiation pathways. These MSCs are cultivated under stringent conditions, ensuring their multipotency and high viability post-thaw. One of the distinguishing features of HMSC-BM compared to MSCs derived from other sources, such as adipose tissue or umbilical cord, is their superior capacity for osteogenic differentiation. This makes them particularly useful in bone biology and orthopedic research, where understanding the molecular mechanisms governing bone formation and repair is crucial. Additionally, HMSC-BM exhibit a robust immunomodulatory profile, which makes them an excellent model for studying immune interactions and inflammatory responses. These unique characteristics also position HMSC-BM as a preferred choice for preclinical studies exploring bone marrow microenvironment, hematopoiesis, and the pathophysiology of bone marrow-related diseases. Each cryovial of HMSC-BM contains a minimum of 1 x 10⁶ cells, with viability rates ranging between 92% to 95%, as determined by the Trypan Blue dye exclusion test. These cells are derived from bone marrow collected from healthy adult donors, all of whom have provided informed consent. To ensure the highest standards, each batch undergoes rigorous quality control testing to assess cell identification, purity, potency, and viability. This thorough testing guarantees that the MSCs meet strict criteria, making them suitable for a wide range of research applications, including cell biology studies, drug discovery, and the investigation of cellular responses to different stimuli. These cells are not intended for therapeutic or in vivo applications, and their use is confined to research purposes in a controlled laboratory environment.
Organism	Human
Tissue	Bone Marrow
Applications	Drug testing, regenerative medicine, disease research

Age	Please inquire
Gender	Please inquire
Ethnicity	Caucasian
Morphology	Well-spread spindle shaped, fibroblast-like morphology for at least within 5 passages. Fewer than 2% cells exhibit spontaneous myofibroblast-like morphology within each passage.
Cell type	Stem cell
Growth properties	Adherent

Citation	Human Mesenchymal Stem Cells, Bone Marrow (HMSC-BM) (Cytion catalog number 300665)
Biosafety level	1
NCBI_TaxID	9606

Antigen expression	A comprehensive panel of markers, including CD73/CD90/CD105 (positive) and CD14/CD34/CD45/HLA-DR (negative), are used in flow cytometry analysis to identify cultivated MSCs (P2-P3) prior to cryopreservation. These markers are recommended by the ISCT MSC committee.
Viruses	Donor is negative for HBV (PCR), Treponema pallidum (PCR), and HIV-1/2 (IFA). Cells are negative for HBV, HCV, HSV1, HSV2, CMV, EBV, HHV6, Toxoplasma gondii, Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, and Ureaplasma parvum.

Culture Medium	Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3
Supplements	Supplement the medium with 10% FBS, 2 ng/mL bFGF
Dissociation Reagent	Trypsin-EDTA
Subculturing	For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C until the cells detach (5-10 minutes). Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO₂, and change the medium every 2-3 days.
Seeding density	1 to 3 x 10⁴ cells/cm²
Fluid renewal	First fluid renewal after 24 hours, then every 2 to 3 days.
Freeze medium	As a cryopreservation medium, we use 80% FBS + 10% basal medium + 10% DMSO to maintain viability, or CM-1 (Cytion catalog number 800100) for superior cryoprotection, preventing unwanted differentiation while preserving pluripotency.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300665-240423P1	Certificate of Analysis	26. Mar. 2026	300665

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

Required products

Required products

Freeze Medium CM-1 - 50 ml

Cryopreservation Media Variants: 50 ml

Cytion’s Freeze Medium CM-1 is a state-of-the-art cryopreservation medium designed to ensure the highest level of cell viability and functionality post-thaw. This versatile medium is suitable for a broad spectrum of cell types, including both human and animal cells, making it an essential tool for diverse research applications. Formulated with a meticulously balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-1 minimizes ice crystal formation and cellular stress during the freezing process, thus preserving cellular integrity.
Key features of Freeze Medium CM-1 include:

Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.

Using CM-1 for Freezing Cells

To use CM-1 for freezing both adherent and suspension cells, follow these steps:

For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step.
Count the cells to ensure they are at the proper concentration.
Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium.
Transfer the resuspended cells into cryovials.
Use a slow-freezing method before transferring the cells to long-term storage.

Method
Description
Steps

❄️
Manual Freezing

A step-by-step method involving gradual temperature reduction to ensure cell viability.

1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes.
2️⃣ Transfer to a -80°C freezer for 24 hours.
3️⃣ Store cells in liquid nitrogen for long-term preservation.

❄️
Using Mr. Frosty

A convenient device that allows for controlled freezing rates without electrical power.

1️⃣ Prepare cells in cryovials with freeze medium.
2️⃣ Place cryovials in Mr. Frosty container.
3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen.

❄️
Controlled-Rate Freezer

A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.

1️⃣ Program the device to gradually decrease the temperature.
2️⃣ Place prepared cells in the freezer.
3️⃣ After the freezing cycle, transfer cells to liquid nitrogen.

Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.

Ingredients

Contains FBS, DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6

Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.

€59.00*

Accutase Cell Detachment Solution - 100 ml

Variants: 100 ml

Accutase Cell Detachment Solution with EDTA and Phenol Red – 100 ml

Accutase is a ready-to-use, sterile-filtered cell detachment solution designed as a gentle alternative to trypsin/EDTA for dissociating adherent mammalian cells from standard tissue culture plasticware and adhesion-coated surfaces. It combines proteolytic and collagenolytic enzyme activity in a balanced salt solution to deliver effective yet controlled dissociation, preserving cell-surface proteins and supporting high post-passage viability and rapid reattachment.

The Accutase formulation is based on Dulbecco’s phosphate-buffered saline (DPBS) with EDTA and phenol red as a visual pH indicator. The enzymes are of non-mammalian and non-bacterial origin, making Accutase particularly well suited to stem cell research, vaccine workflows, and any application where animal
- or microbially-derived contaminants must be minimised. The solution auto-inhibits at 37 °C, so no neutralising reagent or serum-containing medium is required after detachment – cells can be transferred directly into fresh medium.

Key Features

Ready-to-use 1x sterile-filtered liquid – no dilution or reconstitution required
Combined proteolytic and collagenolytic enzyme activity for gentle dissociation
Each batch standardised to a defined dissociation activity for lot-to-lot consistency
Non-mammalian and non-bacterial enzyme origin
Auto-inhibits at 37 °C – no neutralising solution needed
Formulated in Dulbecco’s PBS with EDTA
Phenol red included as visual pH indicator
pH 6.8 – 7.8

Typical Applications
Accutase gently dissociates a wide variety of adherent and sensitive cell types, including human embryonic stem cells (hESCs), human induced pluripotent stem cells (iPSCs), neural stem cells, primary neurons, and routinely cultured adherent lines such as HeLa, HEK 293, CHO, MDCK, Vero, NIH/3T3, BHK-21 and A549. Typical use cases include:

Routine subculture and passaging of adherent mammalian cells
Gentle single-cell dissociation of hESCs, iPSCs and other sensitive lines
Sample preparation for flow cytometry and FACS analysis
Analysis of cell-surface markers where epitope integrity matters
Cell migration, proliferation and apoptosis assays
Quiescence assays by serum starvation and oncogene transfection studies
Tumor cell and neural crest cell migration assays
Production scale-up in bioreactor workflows

For routine work, apply approximately 10 ml of Accutase per 75 cm2 of culture surface and incubate for 5–10 minutes at room temperature. The optimal incubation time should be determined for each cell line and should not exceed one hour. Prior to addition, rinse the cell layer with a Ca2+/Mg2+-free salt solution such as DPBS without calcium and magnesium to remove residual serum and divalent cations.

Handling & Storage
Store the unopened bottle frozen at -15 °C or below. Thaw either at room temperature or overnight at +2 °C to +8 °C. Do not thaw Accutase in a 37 °C water bath, as elevated temperatures reduce enzyme activity. After thawing, the solution can be stored for up to 2 months at +2 °C to +8 °C; do not store at room temperature. Do not pre-warm the reagent to 37 °C before application – add it directly to washed cells at room temperature. For long-term shelf life, single-use aliquoting is recommended to avoid repeated thaw cycles. Always work under aseptic conditions.

Quality
Manufactured under strict quality standards. Each batch of Accutase is sterile-filtered and tested for sterility, pH, appearance and dissociation activity to ensure consistent, reproducible performance from lot to lot.

Product Specifications

Specification
Detail

Product typeCell detachment / dissociation reagent
FormatSterile-filtered liquid, ready-to-use
Volume100 ml
Working concentration1x (ready-to-use)
Enzyme activityCombined proteolytic and collagenolytic
Enzyme originNon-mammalian and non-bacterial
Buffer systemDulbecco’s PBS with EDTA
pH indicatorPhenol red
pH range6.8 – 7.8
AppearanceClear, pale red to orange solution
Storage temperature-15 °C or below
Stability after thawingUp to 2 months at +2 °C to +8 °C
Recommended use volume~10 ml per 75 cm2 culture surface
Typical incubation time5 – 10 minutes at room temperature
Shipping conditionsFrozen on dry ice
Intended useFor research use and further manufacturing only

Formulation (Composition per Liter)

Component
Concentration (mg/L)

Inorganic Salts

Sodium chloride (NaCl)8000.00
Disodium hydrogen phosphate (Na2HPO4)1150.00
Potassium chloride (KCl)200.00
Potassium dihydrogen phosphate (KH2PO4)200.00

Other Components

EDTA · 4Na (tetrasodium EDTA)220.00
Phenol red3.00
Proprietary enzyme blend (proteolytic and collagenolytic activity)1x

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

€75.00*

Antibiotic/Antimycotic Solution (100x)

Product Overview
Volume: 100 ml
Storage: ≤-15°C
Sterility: Sterile-filtered
Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations.
Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures.
Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€45.00*

PBS

Phosphate-Buffered Saline (PBS) Solution
Phosphate-buffered saline (PBS) is a widely used buffer solution in biological and chemical research. It plays a crucial role in maintaining the pH balance and osmolarity during various experimental procedures, including tissue processing and cell culture. Our PBS solution is meticulously formulated with high-purity ingredients to ensure stability and reliability in every experiment. The osmolarity and ion concentrations of our PBS closely mimic those of the human body, making it isotonic and non-toxic to most cells.

Composition of Our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains:

8000 mg/L Sodium chloride (NaCl)
200 mg/L Potassium chloride (KCl)
1150 mg/L Sodium phosphate dibasic anhydrous (Na2HPO4)
200 mg/L Potassium phosphate monobasic anhydrous (KH2PO4)

This composition ensures an optimal pH and ionic balance, suitable for a wide range of biological applications.

Applications of Our PBS Solution
Our PBS solution is ideal for various applications in biological research. Its isotonic and non-toxic properties make it suitable for substance dilution and cell container rinsing. PBS solutions containing EDTA are effective for disengaging attached and clumped cells. However, divalent metals such as zinc should not be added to PBS, as this can cause precipitation. In such cases, Good's buffers are recommended. Additionally, our PBS solution is an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, including SARS-CoV-2.

Quality Control

Sterile-filtered

Storage and Shelf Life

Store at +2°C to +25°C, protected from light.
Once opened, store at 2°C to 25°C and use within 24 months.

Shipping Conditions

Ambient temperature

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.

Composition

Category
Components
Concentration (mg/L)

Salts
Potassium chloride
200

Potassium phosphate monobasic anhydrous
200

Sodium chloride
8000

Sodium phosphate dibasic anhydrous
1150

€20.00*