H9c2(2-1) Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€430.00*

Product number: 305203

Description	H9c2(2-1) cells, derived from the ventricular myoblasts of embryonic BD1X rat hearts, are a subclone of the original H9 cell line established in the early 1990s. These cells are immortalized myoblasts that are commonly used in vitro to study cardiac metabolism, physiology, and pathophysiology, including myocardial ischemia, hypertrophy, and apoptosis mechanisms. Phenotypically, H9c2 cells exhibit characteristics of skeletal muscle but retain the ability to adopt a cardiac muscle phenotype under specific experimental conditions, such as differentiation induced by retinoic acid or other agents. This flexibility makes them a valuable model for investigating cardiac muscle behavior in response to various physiological and pharmacological stimuli. Genetically, H9c2 cells are diploid, facilitating their use in genetic studies, where maintaining a stable karyotype is crucial. Research employing H9c2(2-1) cells has contributed significantly to understanding cellular responses to oxidative stress, mitochondrial dysfunction, and the protective roles of various pharmacological agents against cardiotoxicity. This cell line remains a cornerstone in cardiomyocyte-related research, offering a reproducible, controlled model to elucidate the complex biological and molecular mechanisms underlying cardiac function and diseases.
Organism	Rat
Tissue	Heart, myocardium
Synonyms	H9c2 (2-1), H9c2, H9C2

Receptors expressed	Acetylcholine, expressed
Protein expression	Myokinase, Creatine Phosphokinase, Myosin

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305203-250325	Certificate of Analysis	23. May. 2025	305203
305203-271222	Certificate of Analysis	23. May. 2025	305203

General information