H22 Cells

€800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305163

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	The H22 cell line is a murine hepatocellular carcinoma cell line derived from liver tumor cells. These cells are commonly used in cancer research to study liver cancer mechanisms, therapeutic interventions, and drug efficacy. H22 cells exhibit typical characteristics of hepatocellular carcinoma, including rapid proliferation, resistance to apoptosis, and the ability to form tumors when injected into suitable animal models. This makes them a valuable tool for in vivo studies aiming to understand tumor growth, metastasis, and the tumor microenvironment in liver cancer. One of the significant advantages of the H22 cell line is its use in immunotherapy research. Since the cells are derived from a murine model, they are particularly useful for studying the interactions between cancer cells and the immune system in a controlled environment. Researchers utilize H22 cells to evaluate the efficacy of various immunotherapeutic agents, including checkpoint inhibitors and cancer vaccines. Additionally, H22 cells are employed in the investigation of liver-specific metabolic pathways and the role of genetic mutations in hepatocellular carcinoma progression. Overall, the H22 cell line serves as a robust model for hepatocellular carcinoma, providing insights into cancer biology and aiding in the development of novel therapeutic strategies. Its relevance to both in vitro and in vivo studies underscores its importance in the field of cancer research.
Organism	Mouse
Tissue	Liver
Disease	Hepatocellular carcinoma
Synonyms	Hepatoma-22, Hepatoma 22

Breed/Subspecies	C3HA
Morphology	Lymphoblast
Growth properties	Suspension

Citation	H22 (Cytion catalog number 305163)
Biosafety level	1
NCBI_TaxID	10090
CellosaurusAccession	CVCL_H613

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Subculturing	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305163-170325	Certificate of Analysis	23. May. 2025	305163
305163-070624	Certificate of Analysis	23. May. 2025	305163
305163-041023	Certificate of Analysis	23. May. 2025	305163
305163-241022	Certificate of Analysis	23. May. 2025	305163

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

H22 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Material Transfer Agreement