RCC-JF Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€800.00*

Product number: 300274

Description	Established from the kidney clear cell carcinoma pT2, pM1, No/GII-III of a 65-years-old female, 1999
Organism	Human
Tissue	Kidney
Disease	Clear cell renal cell carcinoma
Synonyms	KTCTL-140, KTCTL140, RCCJF

Protein expression	IL8
Mutational profile	IL8 RS1126647 3-UTR SNP T>T

Culture Medium	McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a)
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	A ratio of 1:2 to 1:3 is recommended
Fluid renewal	1 to 2 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,x CSF1PO: 10,13 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 7,8 TH01: 07. Sep TPOX: 8 vWA: 18 D3S1358: 17 D21S11: 28,30.2 D18S51: 13,15 Penta E: 7,13 Penta D: 10,13 D8S1179: 12,14 FGA: 18,25
HLA alleles	A: '03:01:01 B: '37:01:01, '51:01:01 C: '06:02:01, '07:02:01 DRB1: '11:01:01, '15:01:01G DQA1: '01:02:01, '05:05:01 DQB1: '03:01:01, '06:02:01 DPB1*: '04:01:01, '13:01:01 E: '01:01:01

Lot Number	Certificate Type	Date	Catalog Number
300274-618	Certificate of Analysis	23. May. 2025	300274

General information