U2OS-ZFN-SNAP-Nup107 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300294

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The U-2 OS-ZFN-SNAP-Nup107 cell line represents a specialized variant of the widely used human osteosarcoma cell line U-2 OS. This particular model has been engineered to express the SNAP-tagged version of the nucleoporin Nup107, a pivotal component of the nuclear pore complex, which is essential for the transport of molecules between the nucleus and the cytoplasm. The integration of the SNAP-tag technology allows for the biorthogonal labeling and visualization of Nup107 in live or fixed cells, providing a powerful tool for studying nucleocytoplasmic transport and nuclear pore complex architecture under physiological conditions. The U-2 OS background offers several advantages, including robust growth rates and a well-characterized karyotype, which support high-throughput screening applications and genomic studies. The ZFN (Zinc Finger Nuclease) technology used in this cell line facilitates targeted genome editing, enhancing the precision with which researchers can investigate the genetic contributions to nuclear transport and other cellular processes. This cell line is particularly useful for studies aimed at elucidating the dynamics and regulation of nuclear pore complexes in cancer biology and cellular physiology. Due to the specialized nature of the U-2 OS-ZFN-SNAP-Nup107 no.294 cell line, it is highly suited for advanced imaging techniques, including super-resolution microscopy, to explore nucleoporin functions at unprecedented detail. It is also a valuable resource for developing therapeutic strategies targeting nuclear transport pathways implicated in various diseases, including cancer. The SNAP-tag component adds versatility for further biochemical and proteomic analyses, making it an indispensable tool in the field of cellular and molecular biology.
Organismo	Human
Tecido	Bone
Doença	Osteosarcoma

Idade	15 years
Gênero	Female
Etnia	Caucasian
Propriedades de crescimento	Adherent

Referência	U-2 OS-ZFN-SNAP-Nup107 (Cytion catalog number 300294)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_B7FM
Depositante	The Ellenberg Lab (EMBL)
Situação em relação aos OGMs	GMO-S1: This human osteosarcoma cell line (U2OS-ZFN-SNAP-Nup107 no.294) contains a ZFN-mediated SNAP-Nup107 gene fusion, supporting selective labeling of the Nup107 nuclear pore subunit. The modification is stably present. This classification applies only within Germany and may differ elsewhere.

Expressão de proteínas	SNAP-Nup107 (nuclear pore complex protein 107, SNAP-tag)

Meio de cultura	McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a)
Suplementos	Supplement the medium with 10% FBS, 3.0 g/L Glucose, stable Glutamine, 2.0 mM Sodium pyruvate, 2.2 g/L NaHCO3, 1% NEAA
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300294-119	Certificado de Análise	23. May. 2025	300294

U2OS-ZFN-SNAP-Nup107 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)