U-87 MG-RFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305702

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	U-87 MG-RFP cells are a fluorescently labeled variant of the human glioblastoma astrocytoma cell line U-87 MG, which was originally derived from a malignant glioma tumor of an adult patient. The parental U-87 MG line is widely used as an in vitro model for glioblastoma due to its well-characterized growth properties, tumorigenic potential, and relevance to high-grade astrocytic tumors. These cells exhibit adherent growth with epithelial-like morphology and are commonly employed to study processes such as cell proliferation, invasion, angiogenesis, and response to hypoxic conditions. In U-87 MG-RFP cells, stable expression of red fluorescent protein (RFP) enables real-time visualization of tumor cell behavior in both in vitro and in vivo systems. This modification facilitates applications such as live-cell imaging, tumor tracking in orthotopic xenograft models, and analysis of invasive growth patterns within brain tissue. U-87 MG-RFP cells are particularly valuable for studying glioblastoma progression, tumor–microenvironment interactions, and evaluating therapeutic strategies using fluorescence-based imaging approaches.
Organismo	Human
Tecido	Brain
Doença	Glioblastoma
Sinônimos	U-87MG, U87 MG, U-87-MG, U87-MG, U-87 MG, U-87, U87, 87 MG, 87MG

Idade	44 years
Gênero	Male
Etnia	Caucasian
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	U87MG-RFP (Cytion catalog number 305702)
Nível de biossegurança	1
NCBI_TaxID	9606
Situação em relação aos OGMs	GMO-S1: This human glioblastoma RFP-tagged cell line (U87MG-RFP) contains a lentiviral construct encoding red fluorescent protein from Aequorea victoria, enabling stable red fluorescence labeling. The modification is stably present. This classification applies only within Germany and may differ elsewhere.

Expressão de proteínas	RFP
Isoenzimas	Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B
Tumorigênico	Yes, in nude mice inoculated subcutaneously with 107 cells

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Densidade de semeadura	1.5 to 2 x 10⁴ cells/cm²
Meio de congelamento	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Número do lote	Tipo de certificado	Data	Número de catálogo
305702-170326	Certificado de Análise	13. Apr. 2026	305702

U-87 MG-RFP Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)