SW620-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 305708

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	SW620-GFP cells are a fluorescently labeled variant of the human colorectal adenocarcinoma cell line SW620, which was originally derived from a metastatic lesion in a lymph node of an adult patient. These cells have been engineered to stably express green fluorescent protein (GFP), enabling direct visualization of cellular morphology, proliferation, and migratory behavior in real time using fluorescence-based imaging techniques. The parental SW620 line is characterized by its metastatic origin and exhibits features associated with advanced colorectal cancer, including enhanced motility, invasive capacity, and altered cell adhesion properties. The expression of GFP in SW620-GFP cells facilitates applications such as live-cell tracking, invasion assays, and in vivo imaging in xenograft models, where tumor growth and dissemination can be monitored non-invasively. These cells retain key molecular characteristics of the parental line, including mutations commonly associated with colorectal cancer progression and dysregulation of signaling pathways such as Wnt/β-catenin and MAPK. As a result, SW620-GFP cells are a valuable tool for studying mechanisms of metastasis, tumor microenvironment interactions, and the evaluation of anti-cancer therapeutics targeting advanced-stage colorectal carcinoma.
Organismo	Human
Tecido	Metastatic
Doença	Colon adenocarcinoma
Local da metástase	Lymph node
Sinônimos	SW620, SW 620, SW.620

Idade	51 years
Gênero	Male
Etnia	Caucasian
Morfologia	Epithelial-like
Propriedades de crescimento	Suspension

Referência	SW620-GFP (Cytion catalog number 305708)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_A9MJ
Situação em relação aos OGMs	GMO-S1: This SW-620 metastatic colorectal line contains a GFP construct for visualization of metastatic behavior. This classification applies only within Germany and may differ elsewhere.

Expressão de proteínas	GFP
Tumorigênico	Yes, in athymic nude mice
Perfil mutacional	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

Meio de cultura	DMEM
Meio de congelamento	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Propriedades "SW620-GFP Cells"

Local da metástase:	Lymph node

Número do lote	Tipo de certificado	Data	Número de catálogo
305708-230326	Certificado de Análise	23. Apr. 2026	305708

SW620-GFP Cells

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Dados biomoleculares

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)