SW527 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 300640

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The SW527 cell line is a human breast carcinoma cell line derived from a Caucasian adult patient. It was established in the early 1970s and has been included in foundational studies characterizing tumorigenicity in immunodeficient models. In one such study, SW527 successfully formed tumors in nude mice following subcutaneous inoculation of 6 × 10⁶ cells, supporting its malignant origin. Histopathological analysis of the resulting tumors showed features consistent with the original human carcinoma, confirming its relevance as a breast cancer model. SW527 has been authenticated as a tumor-derived line of G6PD type B, a classification that helps exclude contamination with HeLa cells, which is a critical concern in historical cell line collections. Despite this, comprehensive molecular or immunological profiling of SW527 appears limited in recent large-scale datasets. Overall, SW527 remains a validated breast carcinoma model, primarily supported by in vivo tumorigenicity data. Additional molecular profiling would be beneficial to broaden its utility in mechanistic or drug discovery research.
Organismo	Human
Tecido	Breast; Mammary gland
Doença	Breast adenocarcinoma
Sinônimos	SW-527, SW 527

Idade	70 years
Gênero	Female
Etnia	Caucasian
Morfologia	Epithelial
Tipo de célula	Epithelial
Propriedades de crescimento	Adherent

Referência	SW527 (Cytion catalog number 300640)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_3799

Perfil mutacional	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

Meio de cultura	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Meio de congelamento	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Número do lote	Tipo de certificado	Data	Número de catálogo
300640-120925	Certificado de Análise	05. Dec. 2025	300640

SW527 Cells

Informações gerais

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Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)