SNU-81 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 540,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 305638

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The SNU-81 cell line is a human colorectal carcinoma model established from a Korean patient. It is part of a collection of 12 colorectal cancer cell lines derived from both primary tumors and metastatic sites, providing a diverse representation of tumor biology. SNU-81 was derived from a primary colorectal adenocarcinoma and exhibits epithelial morphology with adherent growth in culture. The cell line expresses carcinoembryonic antigen (CEA), which is secreted into the culture supernatant, reflecting typical colorectal tumor characteristics. At the molecular level, SNU-81 has undergone extensive genetic characterization. It harbors a mutation in the TP53 tumor suppressor gene, a common event in colorectal carcinogenesis, typically associated with later stages of tumor progression. Additionally, mutations in the APC gene were identified, implicating disruption of Wnt/β-catenin signaling, a hallmark of colorectal cancer development. No activating mutations were detected in the K-ras2 gene for this line. Alterations in cell cycle regulators, such as hypermethylation of the p16 gene, were also observed, further supporting the cell line's utility in studying genetic and epigenetic mechanisms driving colorectal cancer. Overall, SNU-81 serves as a well-defined in vitro model for exploring tumor suppressor gene function, oncogenic pathway regulation, and response to targeted therapies in colorectal cancer research.
Organismo	Human
Tecido	Colon
Doença	Adenocarcinoma
Sinônimos	SNU81, NCI-SNU-81

Idade	53 years
Gênero	Male
Etnia	Korean
Morfologia	Epithelial-like
Tipo de célula	Epithelial
Propriedades de crescimento	Adherent, monolayer

Referência	SNU-81 (Cytion catalog number 305638)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_5098

Perfil mutacional	Mutation: APC, Simple, p.Ser1392Ter (c.4175C>A), Heterozygous; Mutation: APC, Simple, p.Arg1450Ter (c.4348C>T), Heterozygous; Mutation: APC, Simple, p.Arg2204Ter (c.6610C>T), Heterozygous; Mutation: FBXW7, Simple, p.Arg479Gln (c.1436G>A), Heterozygous; Mutation: KRAS, Simple, p.Ala146Thr (c.436G>A), Heterozygous; Mutation: PTEN, Simple, p.Arg130Gln (c.389G>A), Heterozygous; Mutation: PTEN, Simple, p.Glu299Ter (c.895G>T), Heterozygous; Mutation: TBX3, Simple, p.Glu111Ter (c.331G>T), Heterozygous; Mutation: TBX3, Simple, c.942-1G>T, Heterozygous; Mutation: TP53, Simple, p.Lys132Thr (c.395A>C), Heterozygous; Mutation: TP53, Simple, p.Arg213Ter (c.637C>T), Heterozygous

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Tempo de duplicação	30 hours
Subcultura	Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305638-061125	Certificado de Análise	11. Dec. 2025	305638

SNU-81 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)