SNU-423 Cells

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305874

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The SNU-423 cell line is a human hepatocellular carcinoma (HCC) model established from a Korean adult patient. It is one of eight HCC cell lines derived from primary liver tumors and characterized for their morphological, genetic, and virological properties. SNU-423 displays substrate adherence and maintains many of the histological features of the original tumor, consistent with hepatocyte-derived epithelial morphology. It exhibits aneuploidy and has a modal chromosome number indicative of chromosomal instability, which is common in HCC-derived lines. At the molecular level, SNU-423 is notable for integration of hepatitis B virus (HBV) DNA within its genome, a characteristic shared by all lines in its cohort, reflecting the high prevalence of HBV-associated liver cancer in Eastern Asia. While some cell lines in the series express HBV transcripts such as HBVx, specific transcript expression in SNU-423 was not reported. Additionally, SNU-423 does not express alpha-fetoprotein (AFP) at either the RNA or protein level, aligning it with a subset of HCCs that lack AFP secretion. It has been used in pharmacogenomic screens such as the LIMORE (Liver Cancer Model Repository), where it contributes to understanding gene-drug associations in liver cancer, including drug response variability potentially linked to HBV status or distinct oncogenic alterations.
Organismo	Human
Tecido	Liver
Doença	Adult hepatocellular carcinoma
Sinônimos	SNU423, NCI-SNU-423

Idade	40 years
Gênero	Male
Etnia	Korean
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	SNU-423 (Cytion catalog number 305874)
Nível de biossegurança	2
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0366

Expressão de antígenos	Blood Type B; Rh +
Perfil mutacional	Mutation: TERT, Simple, c.1-124C>T (c.228C>T) (C228T), Unspecified, Note=In promoter. Mutation, TP53, Simple, c.376-2A>G, Unspecified, Note=Splice acceptor mutation
Cariótipo	Aneuploid; modal number = 79

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% heat-inactivated FBS
Reagente de dissociação	Accutase
Tempo de duplicação	72 hours
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305874-301025	Certificado de Análise	05. Dec. 2025	305874

SNU-423 Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)

Organismo	Human
Tecido	Stomach
Doença	Gastric signet ring cell adenocarcinoma