SNU-387 Cells

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305124

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The SNU-387 cell line is derived from a human hepatocellular carcinoma (HCC) and is widely utilized in liver cancer research. This cell line provides a valuable model for studying the molecular and cellular mechanisms of hepatocarcinogenesis, tumor progression, and therapeutic responses. Hepatocellular carcinoma is one of the most common and lethal forms of liver cancer, making cell lines like SNU-387 essential for advancing our understanding of the disease and developing effective treatments. SNU-387 cells exhibit an epithelial morphology and express markers typical of liver cancer, such as alpha-fetoprotein (AFP) and hepatocyte-specific antigens. They are characterized by genetic and epigenetic alterations common in HCC, including mutations in key oncogenes and tumor suppressor genes. Researchers use SNU-387 cells to investigate signaling pathways involved in liver cancer, such as the Wnt/β-catenin, PI3K/Akt, and MAPK pathways. These cells are also employed in high-throughput drug screening assays and preclinical testing of chemotherapeutic agents and targeted therapies. Additionally, SNU-387 cells are used to study the mechanisms of drug resistance and to develop strategies to overcome it. The relevance of the SNU-387 cell line in hepatocellular carcinoma research highlights its importance in advancing our knowledge of liver cancer biology and in the development of new therapeutic approaches for HCC patients.
Organismo	Human
Tecido	Liver
Doença	Adult hepatocellular carcinoma
Sinônimos	SNU387, NCI-SNU-387

Idade	41 years
Gênero	Female
Etnia	Asian
Morfologia	Epithelial
Propriedades de crescimento	Adherent

Referência	SNU-387 (Cytion catalog number 305124)
Nível de biossegurança	2
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0250

Expressão de antígenos	Blood Type O, Rh +
Vírus	HBV

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Tempo de duplicação	61 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305124-100924	Certificado de Análise	26. May. 2025	305124

SNU-387 Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)

Organismo	Human
Tecido	Stomach
Doença	Gastric signet ring cell adenocarcinoma