SK-MEL-5 Cells

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300157

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	This is one of a very extensive series of melanoma lines which have been isolated by T. Takahashi and associates. The lines served as source of target cells for the detection of melanoma specific antibody in patients with this disease.
Organismo	Human
Tecido	Skin
Doença	Melanoma
Local da metástase	Axillary lymph node
Aplicações	Melanoma research; skin cancer biology; melanoma drug sensitivity; checkpoint inhibitor evaluation; MAPK/ERK pathway studies; cell migration and invasion; xenograft models; NK cell and T cell cytotoxicity assays (target cells)
Sinônimos	SK-Mel-5, SK MEL 5, SK.MEL.5, SK-MEL5, SKMel-5, SKMEL-5, SKMEL5, SKMel5, SKmel5, AA-Mel

Idade	24 years
Gênero	Female
Etnia	Caucasian
Morfologia	Stellate
Tipo de célula	Melanocyte/melanoma cells
Propriedades de crescimento	Adherent

Referência	SK-MEL-5 (Cytion catalog number 300157)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0527
Situação em relação aos OGMs	No genetic modification; wildtype melanoma cell line isolated by T. Takahashi et al.

Expressão de proteínas	P53 positive
Isoenzimas	PGM1, 1-2, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0860
Tumorigênico	Yes, in nude mice, forms malignant melanoma
Produtos	Melanin

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Tempo de duplicação	approx. 24 to 36 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Proporção de divisão	1 to 3
Densidade de semeadura	1 x 10⁴ cells/cm²
Renovação de fluidos	2 to 3 times per week
Recuperação pós-degelo	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300157-170125	Certificado de Análise	23. May. 2025	300157

SK-MEL-5 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)

Organismo	Human
Tecido	Breast, mammary gland
Doença	Invasive ductal carcinoma

Organismo	Human
Tecido	Lung
Doença	Adenocarcinoma (grade III)