SCC-4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305384

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	SCC-4 is a human tongue squamous cell carcinoma (SCC) cell line widely used in cancer research to explore mechanisms of oral cancer progression, apoptosis, and response to chemotherapeutic agents. Oral squamous cell carcinoma is a common malignancy in the oral cavity and is often linked to lifestyle factors such as tobacco use and alcohol consumption. SCC-4 cells are characterized by their aggressive nature and are used to model tumor behavior and treatment resistance in vitro. Studies using SCC-4 have shown that several compounds, such as rhein, emodin, and berberine, induce apoptosis through both intrinsic (mitochondria-dependent) and extrinsic (death receptor-mediated) pathways. Rhein induces S-phase cell cycle arrest and apoptosis through endoplasmic reticulum stress, ROS generation, and mitochondrial dysfunction, triggering the activation of caspase-8, -9, and -3. Similarly, emodin was shown to cause G2/M-phase arrest and induce apoptosis by disrupting mitochondrial membrane potential and promoting cytochrome c release. Berberine also induces apoptosis in SCC-4 cells by enhancing ROS production, increasing intracellular Ca2+, and decreasing mitochondrial membrane potential, thereby activating caspase-9 and caspase-3 pathways. These findings demonstrate that SCC-4 is an effective model for studying the molecular mechanisms of apoptosis in response to potential anticancer agents, providing insight into therapeutic strategies targeting oral squamous cell carcinoma.
Organismo	Human
Tecido	Tongue
Doença	Squamous cell carcinoma
Sinônimos	SCC 4, SCC4

Idade	55 years
Gênero	Male
Etnia	Caucasian
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	SCC-4 (Cytion catalog number 305384)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1684

Perfil mutacional	Mutation: TP53, p.Pro151Ser (c.451C>T)

Meio de cultura	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suplementos	Supplement the medium with 10% FBS and 400 ng/mL hydrocortisone
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305384-021024	Certificado de Análise	05. Dec. 2025	305384

SCC-4 Cells

Informações gerais

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Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)