RF/6A Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 305150

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	RF/6A is a rhesus macaque (Macaca mulatta) retinal choroidal endothelial cell line established from fetal choroid and retina tissue. The line is registered in Cellosaurus as CVCL_4552 and grows as an adherent monolayer with epithelial-like morphology. RF/6A cells retain key endothelial characteristics including expression of Factor VIII (von Willebrand factor), fibronectin, and Weibel-Palade granules detectable by electron microscopy — the latter confirming their endothelial identity. The line was originally established for studies of retinal and choroidal vascularization and has been widely adopted as a primate endothelial model for ocular angiogenesis research. RF/6A is applicable in ocular angiogenesis research, studies of retinal and choroidal vascularization, evaluation of anti-angiogenic agents (VEGF inhibitors, bevacizumab, ranibizumab), age-related macular degeneration (AMD) modeling, diabetic retinopathy biology, and assessment of vascular permeability in the ocular microenvironment. The non-human primate (NHP) origin makes RF/6A closer to human retinal vascular biology than rodent endothelial models, particularly for studies involving primate-specific VEGF isoform responses and ocular pharmacology. The line is commonly used in tube formation assays, migration assays, and VEGF-stimulation experiments. RF/6A is maintained as an adherent culture in EMEM supplemented with 10% FBS and 1% NEAA at 37°C in a humidified 5% CO₂ atmosphere. Cells are subcultured with Accutase at 70–80% confluency to prevent contact inhibition and loss of endothelial phenotype. Split ratio 1:3 to 1:5, seeding density 1–2 × 10⁴ cells/cm². Medium renewed 2–3 times per week.
Organismo	Rhesus macaque
Tecido	Choroid, retina
Doença	Normal retinal choroidal endothelium (fetal; non-tumorigenic)
Local da metástase	Not applicable (normal fetal retinal choroidal endothelial cell line)
Aplicações	Ocular angiogenesis research; retinal and choroidal vascularization; anti-VEGF therapy evaluation (bevacizumab, ranibizumab); AMD and diabetic retinopathy modeling; tube formation assays; vascular permeability; NHP primate retinal endothelial model

Idade	Fetus
Gênero	Sex unspecified
Etnia	Not applicable (non-human primate cell line; Macaca mulatta)
Morfologia	Epithelial-like
Tipo de célula	Endothelial cells
Propriedades de crescimento	Adherent

Referência	RF/6A (Cytion catalog number 305150)
Nível de biossegurança	1
NCBI_TaxID	9544
Número de acesso do Cellosaurus	CVCL_4552
Situação em relação aos OGMs	No genetic modification; wildtype rhesus macaque fetal retinal choroidal endothelial cell line

Expressão de proteínas	Factor Ⅷ, Fibronectin

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Tempo de duplicação	approx. 24 to 36 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Proporção de divisão	1 to 5
Densidade de semeadura	1 to 2 × 10⁴ cells/cm²
Renovação de fluidos	2 to 3 times per week
Recuperação pós-degelo	After thawing, plate the cells at 5 × 10⁴ cells/cm² and allow at least 24 hours for adherence before the first medium change. Do not allow cultures to reach full confluency as contact inhibition may reduce endothelial phenotype.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305150-160223	Certificado de Análise	23. May. 2025	305150
305150-160223SF	Certificado de Análise	23. May. 2025	305150
305150-180625	Certificado de Análise	18. Aug. 2025	305150
305150-260723	Certificado de Análise	22. Jan. 2026	305150

RF/6A Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)