KYSE520 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305449

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The KYSE520 cell line is a human esophageal squamous cell carcinoma (ESCC) model derived from a primary tumor. It is moderately differentiated and has been instrumental in investigating epithelial-mesenchymal plasticity (EMP) in esophageal cancer. KYSE520 cells exhibit heterogeneity, consisting of both epithelial-like (CD44v+) and mesenchymal-like (CD44v−) subpopulations. These two populations are capable of interconversion, reflecting a dynamic EMP process. This property makes KYSE520 an excellent model for studying cancer stem cell traits and chemoresistance mechanisms in ESCC. Genetically, KYSE520 cells display notable epigenetic regulation. The promoter region of the JAM3 gene, a tumor suppressor, is unmethylated in these cells, allowing its expression. JAM3 plays a role in regulating cell proliferation, migration, and invasion through Wnt/β-catenin signaling. The maintenance of JAM3 expression in KYSE520 has been linked to the suppression of aggressive cancer phenotypes. In therapeutic research, KYSE520 cells have been used to explore the role of fibroblast growth factor receptor-like 1 (FGFRL1). Studies have shown that FGFRL1-deficient KYSE520 cells exhibit reduced tumor growth and motility, alongside a decrease in matrix metalloproteinase-1 (MMP-1) and fibroblast growth factor binding protein 1 (FGFBP1) expression. These findings underscore the importance of FGFRL1 in tumorigenesis and suggest potential therapeutic targets. Additionally, the EMP dynamics and associated molecular pathways in KYSE520 cells provide insights into ESCC progression and resistance mechanisms, contributing to the development of targeted treatments.
Organismo	Human
Tecido	Esophagus
Doença	Squamous cell carcinoma
Sinônimos	KYSE 520, KYSE-520, Kyse520, KYSE0520

Idade	58 years
Gênero	Female
Etnia	Japanese
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent, monolayer

Referência	KYSE520 (Cytion catalog number 305449)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1355

Oncogenes	TP53, MYC
Perfil mutacional	Mutation: TP53, c.376-2A>T, Splice acceptor mutation

Meio de cultura	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) + RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a); 1:1 mixture
Suplementos	Supplement the medium with 2% FBS
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidade de semeadura	0.6 - 1.2 x 10⁴ cells/cm²
Renovação de fluidos	2 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305449-020125	Certificado de Análise	05. Mar. 2026	305449
305449-170426	Certificado de Análise	15. May. 2026	305449

KYSE520 Cells

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Dados biomoleculares

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)