KMS-12-PE Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300286

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The KMS-12-PE cell line, established from the pleural effusion of the same patient, differs significantly from KMS-12-BM in several aspects. KMS-12-PE cells represent a more terminally differentiated plasma cell stage, as indicated by the absence of CD20 but continued expression of CD38 and PCA-1. A striking feature of KMS-12-PE is its ability to ectopically produce and secrete a salivary type of amylase, both in the patient’s pleural effusion and in culture, making it unique among human myeloma cell lines. This phenomenon is associated with a chromosomal deletion near the region where the amylase gene is located, specifically del(1)(p22→pter), observed in a significant proportion of KMS-12-PE cells. Despite these distinct differences, both KMS-12-PE and KMS-12-BM share the same clonal marker, the translocation t(11;14)(q13;q32), which is common in myeloma cases. However, KMS-12-PE cells show fewer chromosomal abnormalities than KMS-12-BM and tend to be hypodiploid. Like KMS-12-BM, KMS-12-PE does not produce immunoglobulins, either in surface or secretory form, even though the cells have well-developed endoplasmic reticulum. The lack of tumorigenicity in both cell lines, despite their aggressive in vitro growth, and their stable long-term proliferation in serum-free medium make them valuable tools for studying myeloma biology, particularly in the context of non-Ig-producing myeloma.
Organismo	Human
Tecido	Pleural effusion
Doença	Multiple Myeloma
Sinônimos	KMS 12 PE, KMS-12_PE, KMS-12PE, KMS12-PE, KMS12PE, Kawasaki Medical School-12-Pleural Effusion

Idade	64 years
Gênero	Female
Etnia	Japanese
Morfologia	Round cells
Tipo de célula	B cell
Propriedades de crescimento	Suspension, single cells and small clusters

Referência	KMS-12-PE (Cytion catalog number 300286)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1333

Antígenos de superfície	CD3 -, CD4 -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD34 -, CD38 +, CD138 +, HLA-DR +, PCA-1 +
Tumorigênico	Not tumorigenic in nude mice
Produtos	No immunoglobulin production
Perfil mutacional	Translocation: t(11;14)(q13;q32)

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Subcultura	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Densidade de semeadura	5 x 10⁵ cells/ml
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300286-041024	Certificado de Análise	23. May. 2025	300286

KMS-12-PE Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)