IMR-32 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 300148

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	IMR-32 is a human neuroblastoma cell line derived from the adrenal medulla of a child diagnosed with neuroblastoma, a malignant tumor originating from neural crest cells. These cells exhibit characteristics of immature neuronal cells, making them a valuable model for studying neuronal differentiation, neuroblastoma pathogenesis, and the molecular mechanisms underlying neurodevelopmental processes. The IMR-32 cells have a high capacity for proliferation and retain the ability to synthesize catecholamines, particularly dopamine and norepinephrine, which are essential neurotransmitters in the nervous system. IMR-32 cells display a diploid karyotype with specific chromosomal aberrations commonly associated with neuroblastoma, such as amplification of the MYCN oncogene. This feature makes them particularly useful for research into the genetic and molecular drivers of neuroblastoma, including MYCN's role in tumorigenesis and progression. Additionally, IMR-32 cells are employed in drug screening assays to evaluate the efficacy and cytotoxicity of potential therapeutic agents targeting neuroblastoma. However, it is crucial to note that these cells are intended solely for in vitro research purposes and are not suitable for any therapeutic or in vivo applications.
Organismo	Human
Tecido	Brain
Doença	Neuroblastoma
Local da metástase	Abdomen
Sinônimos	IMR 32, IMR32, Institute for Medical Research-32, GM03320, GM3320C, GM03320D, AG03320, AG3320

Idade	13 months
Gênero	Male
Etnia	Caucasian
Morfologia	Fibroblast-like
Tipo de célula	Neuroblast
Propriedades de crescimento	Adherent

Referência	IMR-32 (Cytion catalog number 300148)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0346

Isoenzimas	G6PD, B
Suscetibilidade ao vírus	Vesicular stomatitis (Indiana), herpes simplex, vaccinia, coxsackievirus B3, poliovirus 3 (poorly)
Resistência a vírus	Echovirus 11
Transcriptase reversa	Negative

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidade de semeadura	1 x 10⁴ cells/cm²
Renovação de fluidos	Every 3 to 5 days
Recuperação pós-degelo	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300148-613	Certificado de Análise	23. May. 2025	300148
300148-040724	Certificado de Análise	23. May. 2025	300148
300148-131123	Certificado de Análise	23. May. 2025	300148

IMR-32 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)