HT-1197 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305800

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	HT-1197 is a human urothelial carcinoma cell line established from a high-grade transitional cell carcinoma of the bladder in an adult male patient. This line was derived from a recurrent tumor after multiple surgical resections and showed aggressive clinical behavior with widespread metastases prior to the patient's death. Morphologically, HT-1197 cells exhibit epithelial features, including the presence of microvilli, tonofibrils, and desmosomes as observed under electron microscopy, indicating their urothelial epithelial origin. These cells are karyotypically distinct with identifiable marker chromosomes and demonstrate the ability to grow in soft agar, a hallmark of anchorage-independent growth, and are tumorigenic in both nude mice and immunosuppressed hamsters. At the molecular level, HT-1197 harbors several key oncogenic mutations commonly associated with bladder cancer. It carries an activating S249C mutation in FGFR3 and an E545K mutation in PIK3CA, both of which are prevalent in the pathogenesis of urothelial bladder carcinoma. Additionally, HT-1197 has a Q61R mutation in NRAS and mutations in the TERT promoter region, suggesting enhanced proliferative capacity and telomerase activity. The TP53 status includes a c.1094A>G alteration, further implicating disruption of cell cycle control and genomic stability. Genomic profiling indicates that HT-1197 belongs to a subset of urothelial cancer cell lines marked by high genomic instability and molecular features consistent with the more aggressive, muscle-invasive subtype of bladder cancer.
Organismo	Human
Tecido	Urinary bladder
Doença	Recurrent bladder carcinoma
Sinônimos	HT 1197, HT1197, HT 1197.T

Idade	44 years
Gênero	Male
Etnia	Caucasian
Propriedades de crescimento	Adherent

Referência	HT-1197 (Cytion catalog number 305800)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1291

Isoenzimas	G6PD, B
Tumorigênico	Yes; Yes, in mice and hamsters
Perfil mutacional	Mutation: NRAS, Simple, p.Gln61Arg (c.182A>G), Unspecified. Mutation, TERT, Simple, c.1-124C>T (c.228C>T) (C228T), Unspecified, Note=In promoter. Mutation, TP53, Simple, p.His365Arg (c.1094A>G), Unspecified

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Tempo de duplicação	61 hours
Renovação de fluidos	twice weekly
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305800-241125	Certificado de Análise	08. Jan. 2026	305800

HT-1197 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)