HPAF-II Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 450,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 305088

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	HPAF-II is a human pancreatic adenocarcinoma cell line derived from an adult patient. This cell line is commonly used in cancer research due to its relevance in studying pancreatic cancer, a highly aggressive and lethal malignancy. HPAF-II cells exhibit epithelial morphology and are known for their ability to form tumors when xenografted into immunocompromised mice, making them a valuable model for in vivo studies of tumor growth, metastasis, and response to therapeutic interventions. Researchers often employ HPAF-II cells to investigate the molecular mechanisms underlying pancreatic cancer progression, including genetic and epigenetic alterations, signal transduction pathways, and interactions with the tumor microenvironment. HPAF-II cells are characterized by specific genetic mutations and alterations that are frequently observed in pancreatic adenocarcinomas. These include mutations in the KRAS gene, which plays a critical role in cell signaling and proliferation, and alterations in tumor suppressor genes such as TP53 and CDKN2A. The cell line also exhibits high levels of mucin production, a feature that contributes to the aggressive nature of pancreatic tumors. Studies utilizing HPAF-II cells have provided significant insights into the biology of pancreatic cancer and have facilitated the development of potential therapeutic strategies aimed at targeting key molecular pathways involved in the disease.
Organismo	Human
Tecido	Pancreas
Doença	Pancreatic ductal adenocarcinoma
Local da metástase	Ascites
Sinônimos	HPAF II, HPAFII, HPAF-2, HPAF2, HPAF/CD18, CD18/HPAF, HPAF-II/CD18, CD-18, CD18, CD 18

Idade	44 years
Gênero	Male
Etnia	European
Morfologia	Epithelial
Propriedades de crescimento	Adherent

Referência	HPAF-II (Cytion catalog number 305088)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0313

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Tempo de duplicação	26 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305088-041023	Certificado de Análise	23. May. 2025	305088
305088-290925	Certificado de Análise	05. Dec. 2025	305088

HPAF-II Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)