HPAC Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 305309

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The HPAC cell line, derived from human pancreatic ductal adenocarcinoma, serves as an essential model for studying pancreatic cancer's molecular and cellular characteristics. Known for its utility in evaluating the impact of various chemotherapeutic agents and signaling pathways, HPAC cells exhibit key features typical of pancreatic cancer, including resistance mechanisms. Recent studies involving HPAC have focused on understanding drug resistance, particularly to erlotinib, a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR). Research has demonstrated that resistance to erlotinib in HPAC cells is associated with significant metabolic alterations, such as changes in phospholipid and amino acid metabolism. Specifically, increased levels of short-chain acylcarnitines and changes in glycerophospholipid profiles have been linked to an elevated metabolic state in erlotinib-resistant HPAC cells. HPAC cells also express matrix metalloproteinases (MMPs), particularly MT1-MMP, which is crucial for their invasive behavior. The Wnt/β-catenin signaling pathway has been implicated in regulating MMP expression, contributing to the cell's migration and invasion potential. The application of compounds like matrine has been shown to inhibit HPAC cell migration by downregulating MT1-MMP through the suppression of Wnt/β-catenin signaling. These attributes highlight HPAC as a pivotal cell line for exploring therapeutic interventions aimed at mitigating pancreatic cancer's aggressive and treatment-resistant nature.
Organismo	Human
Tecido	Pancreas
Doença	Adenocarcinoma
Sinônimos	Hpac

Idade	64 years
Gênero	Female
Etnia	Caucasian
Morfologia	Epithelial-like
Tipo de célula	Pancreatic ductal cell
Propriedades de crescimento	Adherent

Referência	HPAC (Cytion catalog number 305309)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_3517

Expressão de proteínas	Genes expressed: keratin positive, vimentin negative, chromogranin A negative Epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid
Tumorigênico	Yes, in athymic mice
Perfil mutacional	Mutation: CDKN2A, p.Glu120Ter (c.358G>T), homozygous; Mutation: KRAS, p.Gly12Asp (c.35G>A); Mutation: TP53

Meio de cultura	DMEM:Ham's F12, 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate (0.002 mg/ml insulin, 0.005 mg/ml transferrin) ITS+, 40 ng/ml hydrocortisone, 10 ng/ml mouse epidermal growth factor (Fisher Scientific cat# CB-40010)
Suplementos	Supplement the medium with 5% FBS
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
305309-031224	Certificado de Análise	21. Jul. 2025	305309
305309-120625	Certificado de Análise	18. Aug. 2025	305309

HPAC Cells

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Dados biomoleculares

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)