HEK293-Rpn11-HTBH Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305719

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Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	Stable HEK293 Cells-Rpn11-HTBH is a stably transfected derivative of the HEK293 (Human Embryonic Kidney 293) cell line, engineered to express a tagged version of Rpn11 (also known as PSMD14 or POH1), the deubiquitinase subunit of the 26S proteasome lid complex. Rpn11 is a Zn²⁺-dependent JAMM-domain deubiquitinase that removes ubiquitin chains from proteasome-bound substrates during proteasomal degradation. The HTBH tag (hexahistidine-TEV-biotin acceptor peptide-hexahistidine) enables affinity purification of Rpn11-containing complexes under native conditions, making this line particularly suited for proteasome complex purification and interactome studies. This cell line is applicable in studies of 26S proteasome biology, ubiquitin-proteasome pathway (UPS) regulation, Rpn11/PSMD14 function in protein quality control, proteasome assembly and dynamics, and proteasome inhibitor mechanism of action. It is also used for affinity purification of native proteasome complexes and as a model for studying deubiquitinase biology in the context of the proteasome. The HTBH tagging system enables highly stringent purification of biotinylated complexes using streptavidin-based pulldowns. Stable HEK293 Cells-Rpn11-HTBH are maintained as an adherent culture in DMEM supplemented with 10% FBS and the appropriate selection antibiotic to maintain transgene expression at 37°C in a humidified 5% CO₂ atmosphere. Cells are subcultured with Accutase at 80–90% confluency (split ratio 1:5 to 1:10). Medium renewed every 2–3 days.
Organismo	Human
Tecido	Kidney
Doença	Transformed/immortalized fetal kidney (HEK293 background; Rpn11-HTBH transgene)
Aplicações	26S proteasome biology; Rpn11/PSMD14 function; ubiquitin-proteasome pathway; proteasome complex purification; deubiquitinase biology; HTBH-tag affinity purification; proteasome interactome studies

Morfologia	Epithelial-like
Tipo de célula	Epithelial cells
Propriedades de crescimento	Adherent

Referência	Stable HEK293 Cells-Rpn11-HTBH (Cytion catalog number 305719)
Nível de biossegurança	1
NCBI_TaxID	9606
Situação em relação aos OGMs	GMO-S1: This HEK293 derivative contains a stably integrated Rpn11-HTBH expression cassette (hexahistidine-TEV-biotin acceptor peptide-hexahistidine tagged Rpn11/PSMD14). This classification applies only within Germany and may differ elsewhere.

Meio de cultura	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Most cells detach in PBS, if necessary add Accutase 5 min RT
Tempo de duplicação	approx. 24 to 36 hours
Subcultura	Remove medium, wash with PBS without calcium and magnesium, cover with Accutase, incubate 8–10 min at RT, resuspend in medium, centrifuge 300×g 3 min, discard supernatant, reseed in fresh medium.
Proporção de divisão	1 to 10
Densidade de semeadura	2 to 4 × 10⁴ cells/cm²
Renovação de fluidos	Every 2 to 3 days
Meio de congelamento	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Número do lote	Tipo de certificado	Data	Número de catálogo
305719-040526	Certificado de Análise	24. Jun. 2026	305719

HEK293-Rpn11-HTBH Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)