EB1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300403

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The EB1 cell line is a human-derived cell line established from biopsy fragments and cell clumps of Burkitt lymphoma. This line was originally cultivated in Eagle's basal medium supplemented with 10% human serum. The unique growth conditions facilitated the development of cells that predominantly grew as free-floating single individuals or doublets. The EB1 cells exhibit a characteristic doubling time of approximately 48 hours, highlighting their rapid proliferation rate, which is a hallmark feature of lymphoblasts. Morphologically, the EB1 cells display uniform altered lymphoblast characteristics, indicating their derivation from lymphoid tissue. The cell line has been utilized extensively in the study of Burkitt's lymphoma, providing insights into the pathology of lymphoid malignancies. It serves as a valuable model for researching the biological behavior of lymphoid cells under various experimental conditions, aiding in the exploration of therapeutic targets and the understanding of lymphoma progression.
Organismo	Human
Tecido	Blood
Doença	Burkitt lymphoma
Sinônimos	EB-1, Epstein-Barr-1

Idade	9 years
Gênero	Female
Etnia	African
Morfologia	Polymorph cells, big nuclei, formation of microvilli
Tipo de célula	B lymphocyte
Propriedades de crescimento	Suspension

Referência	EB1 (Cytion catalog number 300403)
Nível de biossegurança	2
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_2027

Isoenzimas	PGM1, ESD1, GLO-1, G6PD, B
Vírus	Contains Herpesvirus
Cariótipo	Chromosome Frequency Distribution 30 cells: 2n = 46. The cell line is aneuploid human female, with chromosome counts in the near dipoidrange. Normal chromosomes N8, N11 and N14 are monosomic, with the remainder of autosomes usually being paired. The x chromosome most often is trisomic. Four marker chromosomes are found. Two of these (markers M1 and M3) involve the reciprocal translocation between chromosomes N8 and N14 associated with most Burkitt's lymphoma cell lines.

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% heat-inactivated FBS
Tempo de duplicação	48 hours
Subcultura	The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Densidade de semeadura	0.1 x 10⁶ cells/ml
Renovação de fluidos	2 to 3 times per week
Recuperação pós-degelo	After thawing, allow the cells to recover from the freezing process for at least 24 hours
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300403-213	Certificado de Análise	23. May. 2025	300403
300403-240225	Certificado de Análise	23. May. 2025	300403

EB1 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)