Capan-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Preços sem impostos, mais custos de envio

cryovial

Número do produto: 300143

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The Capan-1 cell line is derived from a human pancreatic adenocarcinoma and was established from the ascitic fluid of a 40-year-old Caucasian male. It was first characterized in 1975 and is particularly noted for its ductal epithelial morphology, which closely resembles that of primary pancreatic tumors. Capan-1 cells are extensively used in research aimed at understanding pancreatic cancer biology, including studies on tumor progression, metastasis, and treatment resistance. This cell line is well-regarded for its ability to produce mucin, a characteristic feature of many pancreatic adenocarcinomas, thus serving as a model for mucinous pancreatic cancer. Genetically, Capan-1 harbors mutations in the KRAS gene, which are typical of pancreatic cancer, as well as alterations in other cancer-related genes such as TP53 and SMAD4. These mutations make the Capan-1 cell line a valuable tool for studying the molecular mechanisms underlying pancreatic cancer and for the preclinical evaluation of new therapeutic agents targeting these pathways. Furthermore, Capan-1 cells are used to study the biology of pancreatic cancer stem cells, offering insights into the behaviors that drive cancer recurrence and resistance to conventional therapies.
Organismo	Human
Tecido	Pancreas
Doença	Ductal adenocarcinoma
Local da metástase	Liver
Sinônimos	CaPan-1, CAPAN-1, Capan 1, CAPAN 1, Capan1, CAPAN1

Idade	40 years
Gênero	Male
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	Capan-1 (Cytion catalog number 300143)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_0237

Expressão de proteínas	P53 negative
Expressão de antígenos	Blood Type A, Rh+
Isoenzimas	Me-2, 1, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, G6PD, B, GLO-1, 1-2, Phenotype Frequency Product: 0.0311
Tumorigênico	Form adenocarcinoma consistent with pancreatic duct carcinoma
Produtos	Mucin
Perfil mutacional	Capan-1 cells carry a homozygous Kras mutation in codon12: GGT(Gly) >GTT(Val)
Cariótipo	(P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker

Meio de cultura	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Tempo de duplicação	60 to 80 hours
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidade de semeadura	2 x 10⁴ cells/cm² will result in a 90% confluent monolayer in about 7 days
Renovação de fluidos	Every 3 days
Recuperação pós-degelo	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300143-080525	Certificado de Análise	21. Jul. 2025	300143
300143-270224	Certificado de Análise	21. Jul. 2025	300143
300143-c822p	Certificado de Análise	23. May. 2025	300143

Capan-1 Cells

Basic details about CaPan-1 cell lines

Properties of the pancreatic adenocarcinoma cell line Capan-1

Specification

Genomics of Capan-1 pancreatic cancer cells

Cell culture practices

CaPan-1 cell quality verification

Certificado de Análise (CoA)