BGM Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 302158

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	BGM (Buffalo Green Monkey) cells are a kidney epithelial cell line derived from the African green monkey, Cercopithecus aethiops. These cells are typically used in virological studies because of their susceptibility to various enteroviruses and other viral pathogens, making them a valuable tool in the study of viral infections and viral-host interactions. Their high permissiveness for viral replication is particularly useful for isolating and propagating enteroviruses, rotaviruses, and adenoviruses, among others. In addition to their use in virology, BGM cells are employed in cytotoxicity testing and vaccine production. They provide a consistent and controlled environment for testing the effects of new drugs and potential vaccines on cellular health and viability. BGM cells are also utilized in genetic studies, particularly in understanding gene expression and signaling pathways involved in viral infection and host response mechanisms. Their robust growth and ease of handling in laboratory settings further contribute to their widespread use in biological research.
Organismo	Vervet monkey
Tecido	Kidney
Aplicações	Isolation of water borne viruses
Sinônimos	Buffalo Green Monkey cells, BGMK, Buffalo Green Monkey Kidney cells

Gênero	Male
Morfologia	Epithelial-like
Propriedades de crescimento	Adherent

Referência	BGM (Cytion catalog number 302158)
Nível de biossegurança	1
NCBI_TaxID	60710
Número de acesso do Cellosaurus	CVCL_4125

Meio de cultura	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
302158-250424	Certificado de Análise	23. May. 2025	302158

BGM Cells

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Dados biomoleculares

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)