B16-F0 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 395,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300308

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The B16-F0 cell line is a murine melanoma cell line derived from the B16 mouse melanoma. This cell line is widely used in cancer research due to its high metastatic potential and ability to form tumors when injected into syngeneic mice. B16-F0 cells are particularly useful for studying the molecular mechanisms underlying melanoma progression and metastasis, as well as for testing the efficacy of anti-cancer drugs and therapeutic interventions in preclinical models. Notably, the B16-F0 cell line is the parent cell line from which other variants, such as B16-F1, B16-F10, and B16-BL6, have been derived through selective procedures aimed at enhancing specific metastatic properties. B16-F0 cells exhibit a typical epithelial morphology and grow adherently in culture. They are known to express various melanoma-associated antigens, making them a valuable tool for immunological studies and for developing melanoma vaccines. Additionally, these cells are often employed in studies involving gene expression, signaling pathways, and the tumor microenvironment. Researchers utilize B16-F0 cells to explore the interactions between melanoma cells and the immune system, particularly focusing on immune evasion and suppression mechanisms. The characterization of B16-F0 and its derived lines provides a comprehensive framework for understanding the invasive and metastatic behaviors of melanoma, with B16-F1, B16-F10, and B16-BL6 each representing stages of increasing metastatic and invasive activity, thereby serving as critical models in the study of cancer progression and therapeutic response.
Organismo	Mouse
Tecido	Skin
Doença	Mouse melanoma
Sinônimos	B16/F0, B16F0

Raça/Subespécie	C57BL/6
Gênero	Male
Morfologia	Mixture of spindle-shaped and epithelial-like cells
Tipo de célula	Epithelial
Propriedades de crescimento	Adherent

Referência	B16-F0 (Cytion catalog number 300308)
Nível de biossegurança	1
NCBI_TaxID	10090
Número de acesso do Cellosaurus	CVCL_0604

Tumorigênico	Yes, in syngeneic mice
Produtos	Melanin

Meio de cultura	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300308-250324	Certificado de Análise	23. May. 2025	300308

B16-F0 Cells

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Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)