WI 38 VA13 subline 2RA Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300421

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The WI-38 VA13 subline 2RA, derived from the historic WI-38 cell line originally sourced from the lung tissue of a 3-month-old fetus, represents a key advancement in cell culture technology. The original WI-38 cell line was crucial in developing vaccines for numerous viral diseases, such as measles, mumps, rubella, and hepatitis A. The VA13 subline 2RA is an immortalized variant of this cell line, achieved through transformation with Simian Virus 40 (SV40), a practice common in the development of immortal cell lines which allows for indefinite cell replication beyond the standard senescence point of about 50 population doublings. The incorporation of SV40 into the WI-38 cells to create the VA13 subline 2RA extends the cells' lifespan, providing a more durable model for long-term experiments. This transformation maintains the fundamental properties of the original diploid cells but alters their lifecycle and growth patterns, enabling sustained growth and facilitating extensive studies that were not possible with the finite lifespan of the parent cell line. This makes the VA13 subline particularly useful in ongoing and extensive research areas, including virology, pharmacology, and genetic research, where extended observation periods are necessary.
Organismo	Human
Tejido	Lung
Sinónimos	WI 38 VA-13 subline 2RA, WI 38VA13 subline 2RA, WI-38 VA13 sub 2 RA, WI38-VA13 subline 2RA, WI38 VA13/2RA, WI38VA13/2RA, VA13 2RA, WI-38 VA13, WI 38 VA 13, WI38-VA13, WI38/VA13, WI38VA13, VA-13, VA13, AG07217, AG7217

Edad	3 months gestation
Género	Female
Origen étnico	Caucasian
Morfología	Epithelial-like
Tipo de célula	Fibroblast
Propiedades de crecimiento	Adherent

Referencia	WI 38 VA13 subline 2RA (Cytion catalog number 300421)
Nivel de bioseguridad	2
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_2759

Isoenzimas	G6PD, B
Virus	Contains Papovavirus
Susceptibilidad a los virus	Herpes simplex, vesicular stomatitis (Indiana), poliovirus 2
Transcriptasa inversa	Negative
Cariotipo	Hyperdiploid, Modal number: 73-78

Medio de cultivo	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidad de siembra	1 x 10⁴ cells/cm²
Renovación de fluidos	1 to 2 times per week
Recuperación tras el deshielo	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300421-050523	Certificado de análisis	23. May. 2025	300421
300421-300525	Certificado de análisis	18. Aug. 2025	300421

WI 38 VA13 subline 2RA Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)