U-CH1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 500.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305885

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The U-CH1 cell line is the first established permanent human chordoma cell model, derived from a recurrent sacral chordoma. Chordomas are rare, slow-growing, locally invasive tumors that originate from notochordal remnants and primarily occur along the axial skeleton. U-CH1 exhibits cytogenetic features characteristic of chordoma, including clonal chromosomal aberrations such as der(1)t(1;22), deletions on chromosomes 4, 5, 6, 9, 10, and 20, and a derivative chromosome 20 resulting from t(10;20). Comparative genomic hybridization revealed recurrent DNA copy number changes in chordomas, particularly losses on 1p and 3p and gains on 7q, 5q, 12q, and 20. The cytogenetic profile of U-CH1 closely mirrors that of its parental tumor, reinforcing its biological relevance. Functionally and molecularly, U-CH1 and other chordoma cell lines display hallmark features of chordoma, including expression of brachyury, a transcription factor considered a key diagnostic marker. U-CH1 also harbors deletions of CDKN2A and lacks p16 protein expression, a recurrent genetic alteration in chordomas. This alteration leads to hyperactivation of the CDK4/6 pathway, rendering U-CH1 sensitive to CDK4/6 inhibitors such as palbociclib. Treatment with palbociclib significantly reduced phosphorylated Rb levels and inhibited proliferation in vitro, indicating that U-CH1 can be a valuable preclinical model for evaluating cell cycle-targeted therapies. The cell line has also been validated through mRNA and protein profiling, confirming its representativeness of primary chordoma tumors in expression and genomic patterns.
Organismo	Human
Tejido	Bone, sacrum
Enfermedad	Sacral chordoma
Sinónimos	UCH-1, UCH1

Edad	56 years
Género	Male
Origen étnico	White
Morfología	Mesenchymal like, with variable vacuoles
Tipo de célula	Chordoma
Propiedades de crecimiento	Adherent

Referencia	U-CH1 (Cytion catalog number 305885)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_4988

Perfil mutacional	Mutation: TP53, Simple, p.Pro72Arg (c.215C>G), Unspecified

Medio de cultivo	IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Tiempo de duplicación	~1 week
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305885-020126	Certificado de análisis	03. Feb. 2026	305885

U-CH1 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)