SNU-5 Cells

USD 540.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305633

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The SNU-5 cell line is a human gastric carcinoma model established from a metastatic lesion. It has been characterized for its molecular abnormalities, particularly those involving the p53 tumor suppressor gene. Studies show that SNU-5 exhibits a deletion of the p53 gene transcript, as determined by the absence of p53 mRNA in Northern blot analyses. This loss was further supported by RNase protection assays and sequencing, which revealed that SNU-5 lacks detectable mutations in the coding regions but fails to express the transcript altogether, indicating a possible regulatory or epigenetic mechanism of gene silencing rather than a structural mutation. Proteomic analyses have provided deeper insights into the molecular characteristics of SNU-5. Large-scale studies have included SNU-5 among a panel of cancer cell lines used to map the human cancer cell line proteome. In this context, SNU-5 contributes to datasets integrating mass spectrometry-based quantification of thousands of proteins. These proteomic datasets have been correlated with transcriptomic, genomic, and phenotypic profiles, offering a comprehensive view of protein expression, post-transcriptional regulation, and drug response characteristics. Such datasets position SNU-5 as a valuable model for investigating gastric cancer biology, especially in the context of metastatic disease and p53 pathway dysregulation.
Organismo	Human
Tejido	Gastric
Enfermedad	Adenocarcinoma
Lugar de metástasis	Ascites
Aplicaciones	3D cell culture, Cancer research
Sinónimos	SNU5, NCI-SNU-5

Edad	33 years
Género	Female
Origen étnico	Korean
Morfología	Lymphoblast-like
Tipo de célula	Lymphoblast
Propiedades de crecimiento	Suspension

Referencia	SNU-5 (Cytion catalog number 305633)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_0078
Estado de los OGM	GMO-S1: This 4T1 carcinoma derivative contains a-Luc reporter construct introduced by lentiviral transduction, enabling bioluminescent tumor monitoring. This classification applies only within Germany and may differ elsewhere.

Perfil mutacional	Mutation: CDKN2A, Simple, p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T), Homozygous; Mutation: TP53, Simple, p.Gly262_Ser269delGlyAsnLeuLeuGlyArgAsnSer (c.784_807del24), Unspecified

Medio de cultivo	IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a)
Suplementos	Supplement the medium with 20% FBS
Reactivo de disociación	Accutase
Tiempo de duplicación	34 hours
Subcultivo	Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305633-121225	Certificado de análisis	22. Jan. 2026	305633

SNU-5 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)