SF188 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305870

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The SF188 cell line is a human glioblastoma multiforme (GBM) model established from a pediatric patient. It is used extensively to study the mechanisms of chemotherapeutic resistance, particularly to alkylating agents such as 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). Compared to other glioma-derived cell lines such as SF126, SF188 exhibits significantly higher resistance to BCNU-induced cytotoxicity and genotoxicity. Specifically, SF188 shows approximately threefold greater resistance in survival assays and 14-fold lower susceptibility to BCNU-induced sister chromatid exchange (SCE), indicating a robust DNA damage tolerance phenotype. The resistance in SF188 is attributed to enhanced DNA repair capacity, especially the rapid and efficient removal of O⁶-alkylguanine adducts. Upon exposure to methylating agents like N-methyl-N-nitrosourea, SF188 cells demonstrate marked removal of O⁶-methylguanine lesions, whereas more sensitive cell lines show minimal repair activity. This efficient lesion repair likely prevents the formation of interstrand crosslinks, thereby maintaining genomic integrity and increasing cell survival. Importantly, SF188 also exhibits a high chromosomal count (modal number 91) and lacks expression of glial fibrillary acidic protein (GFAP), confirming its poorly differentiated glioma origin and making it an excellent model for studying the interplay between DNA repair and chemoresistance in high-grade gliomas.
Organismo	Human
Tejido	Brain, right frontal lobe
Enfermedad	Glioblastoma
Sinónimos	SF-188, SF 188

Edad	8 years
Género	Male
Propiedades de crecimiento	Adherent

Referencia	SF188 (Cytion catalog number 305870)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_6948

Perfil mutacional	Mutation: TP53, Simple, p.Gly266Glu (c.797G>A), Homozygous (PubMed=9614553, PubMed=10416987).

Medio de cultivo	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reactivo de disociación	Accutase
Tiempo de duplicación	26 hours
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densidad de siembra	2 to 4 x 10⁴ cells/cm²
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305870-060526	Certificado de análisis	24. Jun. 2026	305870

SF188 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)