SF126 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300608

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The SF126 cell line is a human glioblastoma cell line, widely utilized in research on brain tumors, particularly in studies exploring the molecular mechanisms of glioblastoma and its response to various treatments. Derived from a patient with glioblastoma multiforme, SF126 cells are known for their aggressive growth and invasive behavior, typical of glioblastomas, making them a crucial model for investigating therapeutic strategies and understanding tumor biology. One of the notable features of SF126 is its use in exploring both apoptosis (programmed cell death) and autophagy, as these processes are central to cancer cell survival and resistance to treatment. SF126 has been extensively studied for its interactions with p53, a tumor suppressor gene frequently mutated in cancers. In SF126, researchers have investigated the effects of wild-type and mutant p53 on cell death mechanisms. It was found that p53 induces both apoptosis and autophagy, with autophagic cell death playing a significant role in p53-dependent cell death. This has implications for therapies targeting autophagic pathways, which may enhance the efficacy of treatments aimed at inducing tumor cell death. Additionally, studies have shown that manipulating autophagy can influence the overall tumor response to p53 activation, offering potential therapeutic angles for glioblastoma treatment. Further research on SF126 has explored its binding properties with opioid peptides, such as β-endorphins, revealing specific binding sites for these molecules. This has provided insights into how glioblastoma cells might interact with endogenous hormones and signaling molecules in the brain, further underscoring the complexity of glioblastoma biology and potential novel therapeutic targets.
Organismo	Human
Tejido	Brain, left frontal lobe
Enfermedad	Glioblastoma
Aplicaciones	cell biology studies of gliomas
Sinónimos	SF-126, SF 126

Edad	50 years
Género	Female
Origen étnico	European
Propiedades de crecimiento	Adherent

Referencia	SF126 (Cytion catalog number 300608)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1688

Tumorigénico	No (tested in athymic mice)
Productos	Procollagen III, forms collagen fibers in vitro (interstitial collagen synthesis)
Estado de ploidía	Aneuploid

Medio de cultivo	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suplementos	Supplement the medium with 10% FBS and 1% NEAA
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300608-080724	Certificado de análisis	23. May. 2025	300608

SF126 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)