NCI-H2087 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305824

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	NCI-H2087 is a human non-small cell lung carcinoma (NSCLC) cell line derived from the metastatic site (specifically, a lymph node) of an adult patient with lung adenocarcinoma. This cell line is epithelial in morphology and is commonly utilized in studies investigating lung cancer pathogenesis, therapeutic responses, and molecular profiling of metastatic adenocarcinomas. It exhibits characteristics consistent with its origin, including expression of epithelial markers and various genetic alterations typical of lung adenocarcinomas. Genetically, NCI-H2087 is known to harbor mutations relevant to oncogenesis and therapy resistance in NSCLC. Most notably, it contains a KRAS mutation, which is associated with constitutive activation of downstream signaling pathways such as MAPK and PI3K-AKT, leading to enhanced cell proliferation and survival. The presence of this mutation makes NCI-H2087 a valuable model for studying KRAS-driven tumorigenesis and for evaluating targeted inhibitors that disrupt KRAS signaling. Additionally, the cell line is p53 mutant, which can contribute to impaired apoptosis and genomic instability, further supporting its utility in preclinical cancer biology and drug screening research.
Organismo	Human
Tejido	Lymph node
Enfermedad	Lung adenocarcinoma
Sinónimos	H2087, H-2087, NCIH2087

Edad	69 years
Género	Male
Origen étnico	Caucasian
Morfología	Epithelial-like and/or rounded
Propiedades de crecimiento	Adherent

Referencia	NCI-H2087 (Cytion catalog number 305824)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1524

Perfil mutacional	Mutation: ATM, Simple, p.Glu848Gln (c.2542G>C), Heterozygous, BRAF, Simple, p.Leu597Val (c.1789C>G), Heterozygous, MYC, Simple, p.Glu54Lys (c.160G>A), Heterozygous, NRAS, Simple, p.Gln61Lys (c.181C>A), Heterozygous, TP53, Simple, p.Val157Phe (c.469G>T), Homozygous

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	51 hours
Reactivo de disociación	Accutase
Densidad de siembra	4 x 10⁴ cells/cm²
Medio de congelación	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305824-101025	Certificado de análisis	05. Dec. 2025	305824

NCI-H2087 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)