KC Cells
Información general
| Descripción | KC (Kc167) is a Drosophila melanogaster embryonic cell line derived from a dorsal closure stage female embryo, registered in Cellosaurus as CVCL_Z833. The line was originally established and has been widely used in Drosophila cell biology, genetic screens, and functional genomics. Kc167 cells have been immortalized with SV40 and grow semi-adherently, forming a mixed population of attached and loosely floating cells. They are among the best-characterized Drosophila cell lines and are fully amenable to RNAi-mediated gene silencing, making them a cornerstone model for genome-wide RNAi screens in invertebrate biology. KC cells are applicable in Drosophila cell biology, genome-wide RNAi and CRISPR screens, signal transduction pathway studies (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB), innate immunity research, and comparative studies of evolutionarily conserved pathways. The fully sequenced and annotated Drosophila genome, combined with extensive public databases of RNAi reagents (e.g., DRSC/TRiP), makes KC cells particularly powerful for unbiased functional genomic discovery. BSL-2 classification applies due to the SV40 immortalization. KC cells are cultured in Schneider's Drosophila medium supplemented with 2 mM Glutamine and 10% FBS. Critically, Drosophila cell lines are cultured at 25°C in ambient air without CO₂ (not 37°C / 5% CO₂ as for mammalian cells). Cells are passaged by gentle resuspension and dilution (semi-adherent growth). Medium is renewed or cells are diluted every 2–3 days. |
|---|---|
| Organismo | Drosophila melanogaster (Fruit fly) |
| Tejido | Embryo |
| Enfermedad | Normal |
| Lugar de metástasis | Embryo (dorsal closure stage) |
| Aplicaciones | Drosophila cell biology; genome-wide RNAi screens; CRISPR screens; signal transduction (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB); innate immunity; invertebrate functional genomics |
| Sinónimos | KC, K C |
Características
| Edad | Dorsal closure stage |
|---|---|
| Género | Female |
| Morfología | Semi-adherent epithelial-like |
| Tipo de célula | Embryonic cells |
| Propiedades de crecimiento | semi-adherent |
Datos normativos
| Referencia | KC (Cytion catalog number 300604) |
|---|---|
| Nivel de bioseguridad | 2 |
| NCBI_TaxID | 7227 |
| N.º de acceso de Cellosaurus | CVCL_Z833 |
| Estado de los OGM | GMO-S2: This Drosophila cell line contains a stably integrated SV40 immortalization cassette. BSL-2 containment is required. This classification applies only within Germany and may differ elsewhere. |
Datos biomoleculares
| Susceptibilidad a los virus | SV40 immortalized |
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Manejo
| Medio de cultivo | Schneider's Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
|---|---|
| Suplementos | Supplement the medium with 2 mM Glutamine and 10% FBS |
| Reactivo de disociación | Not required (semi-adherent; resuspend gently) |
| Tiempo de duplicación | approx. 24 to 48 hours |
| Subcultivo | Gently pipette the culture to resuspend semi-adherent cells. Transfer an appropriate volume to a new flask and add fresh pre-warmed Schneider's medium. Dilute to a density of 5 × 10⁵ to 1 × 10⁶ cells/ml. Incubate at 25°C in ambient air without CO₂. |
| Relación de división | 1 to 3 |
| Densidad de siembra | 5 × 10⁵ to 1 × 10⁶ cells/ml |
| Renovación de fluidos | Every 2 to 3 days |
| Medio de congelación | As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Descongelación y cultivo de células |
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| Atmósfera de incubación | 25°C, ambient air (no CO₂ required). |
| Condiciones de envío | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Condiciones de almacenamiento | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |