Jiyoye Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300366

Ficha de datos de seguridad

Ficha del producto

Descripción	The Jiyoye cell line is an extensively studied model derived from a human Burkitt lymphoma. Burkitt lymphoma is a type of non-Hodgkin lymphoma that predominantly affects B cells, and the Jiyoye cell line retains many of the key characteristics of this malignancy. The cells exhibit the typical chromosomal translocation between the c-MYC gene and immunoglobulin gene loci, which is a hallmark of Burkitt lymphoma. This translocation leads to the overexpression of the c-MYC oncogene, driving the proliferative and aggressive nature of the tumor cells. As such, the Jiyoye cell line is an invaluable tool for studying the molecular and genetic mechanisms underlying lymphomagenesis, especially in the context of MYC-driven cancers. Jiyoye cells grow in suspension and are characterized by their high proliferation rate, which makes them suitable for a variety of experimental applications, including drug screening, gene expression studies, and apoptosis assays. The cell line is also frequently used in research focusing on Epstein-Barr virus (EBV), as Burkitt lymphoma cells, including Jiyoye, often harbor this virus, which is implicated in the pathogenesis of the disease. This makes Jiyoye particularly useful for investigating the interplay between viral oncogenes and cellular pathways in B cell malignancies. Given its origin and characteristics, the Jiyoye cell line is a critical model for oncological research, particularly in understanding the pathophysiology of B cell lymphomas.
Organismo	Human
Tejido	Lymphatic System
Enfermedad	B-cell Non-Hodgkin-Lymphoma
Lugar de metástasis	B-Lymphocyte
Aplicaciones	Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, haplotype standard.
Sinónimos	JIYOYE, Jijoye, JIJOYE, P-2003, P3 (Jiyoye), P-3-Jijoye, P3-Jiyoye, P-3J, P3J, Jiyoye(P-2003), Jiyoye (P-2003), JiyoyeP-2003, OB2, GM04678

Edad	7 years
Género	Male
Origen étnico	African
Tipo de célula	B lymphocyte
Propiedades de crecimiento	Suspension

Referencia	Jiyoye (Cytion catalog number 300366)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1317

Expresión de antígenos	CD10+, CD19+
Cariotipo	46, hypodiploid

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Subcultivo	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Densidad de siembra	3 x 10⁵ cells/ml
Renovación de fluidos	2 to 3 times per week
Recuperación tras el deshielo	Fast (48 hours)
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Jiyoye Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular