HROC348 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

Cytion GmbH

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300719

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	HROC348 is a human colorectal carcinoma cell line derived from a primary tumor resected from an adult male patient diagnosed with sigmoid colon cancer. The tumor was classified as a moderately advanced adenocarcinoma (T3, G3, N2), indicating significant local invasion and lymph node involvement, consistent with aggressive tumor behavior. The carcinoma originated in the sigmoid colon, a common anatomical site for sporadic colorectal cancer, and presented with microsatellite stability (MSS), which aligns it with the chromosomal instability (CIN) subtype rather than the MSI-high hypermutated class of colorectal tumors. Molecular profiling of HROC348 shows wild-type status for both KRAS and BRAF, suggesting the absence of common activating mutations in these genes that are frequently implicated in colorectal cancer progression and therapy resistance. This molecular background makes HROC348 particularly suitable for studies focused on non-mutated RAS/RAF signaling and its implications in tumor growth, therapeutic response, and resistance mechanisms. The cell line does not display the CpG island methylator phenotype (CIMP), further supporting its classification within the conventional (non-hypermutated) colorectal cancer subgroup. Clinically, the tumor was positive for lymph node metastasis (LN_pos = 2), but distant metastasis (M) was noted only once, and no right-sided colon involvement was recorded, consistent with a left-sided colorectal cancer profile. These features, combined with the MSS status and molecular markers, position HROC348 as a representative model for studying left-sided, KRAS/BRAF wild-type, microsatellite-stable colorectal adenocarcinoma. It also offers translational value for preclinical testing of targeted therapies and immune-modulatory agents in MSS tumors, which are typically less responsive to immune checkpoint blockade.
Organismo	Human
Tejido	Sigmoid colon
Enfermedad	Carcinoma
Lugar de metástasis	Not reported (primary sigmoid colon adenocarcinoma; no confirmed distant metastasis at time of sampling)
Aplicaciones	Colorectal cancer research; KRAS/BRAF wild-type MSS CRC biology; left-sided colorectal cancer modeling; drug sensitivity in non-mutated RAS/RAF tumors; HROC Linnebacher biobank studies; CRC immunotherapy evaluation; preclinical oncology

Edad	77 years
Género	Male
Origen étnico	Caucasian
Morfología	Epithelial-like
Tipo de célula	Epithelial cells
Propiedades de crecimiento	Adherent

Referencia	HROC348 (Cytion catalog number 300719)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	Not assigned
Estado de los OGM	No genetic modification; wildtype patient-derived CRC cell line from the HROC Linnebacher biobank. KRAS wild-type, BRAF wild-type, MSS, CIMP-negative.

Estado de MSI	MSS

Medio de cultivo	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300719-280325	Certificado de análisis	23. May. 2025	300719

HROC348 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)