HK-CRISPR-Nup62-mEGFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300659

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The HK-CRISPR-Nup62-mEGFP cell line is a human cell model with the Nup62 gene tagged with monomeric Enhanced Green Fluorescent Protein (mEGFP). This modification allows for real-time visualization of Nup62 within the nuclear envelope, aiding studies on nuclear transport, NPC assembly, and dynamics. Precise genome editing was achieved using CRISPR-Cas9 technology, making it a reliable model for investigating Nup62's role in cellular processes. This cell line is valuable for research in nucleocytoplasmic transport, cell cycle regulation, and nuclear architecture. The fluorescent tagging of Nup62 enables high-resolution imaging and live-cell tracking, facilitating fluorescence microscopy and other imaging techniques. Researchers can explore the molecular mechanisms of nuclear-cytoplasmic exchange and the role of Nup62 in diseases such as cancer and neurodegenerative disorders.
Organismo	Human
Tejido	Endocervix
Enfermedad	Adenocarcinoma

Edad	30 years
Género	Female
Origen étnico	African American
Morfología	Epithelial-like cells with mosaic stone shape
Propiedades de crecimiento	Adherent

Referencia	HK-CRISPR-Nup62-mEGFP (Cytion catalog number 300659)
Nivel de bioseguridad	1
NCBI_TaxID	9606
Depositante	The Ellenberg Lab (EMBL)
Estado de los OGM	GMO-S1: This HeLa Kyoto line contains an mEGFP-tagged Nup62 generated via CRISPR, enabling imaging of central channel nuclear pore components. This classification applies only within Germany and may differ elsewhere.

Expresión de proteínas	Nup62, mEGFP-tag

Medio de cultivo	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300659-161123	Certificado de análisis	23. May. 2025	300659

HK-CRISPR-Nup62-mEGFP Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)