HEL 92.1.7 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 300462

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The HEL 92.1.7 cell line exhibits the capacity for spontaneous differentiation into erythroblast-like cells, mimicking some aspects of erythroid maturation in vitro. This characteristic makes them particularly useful for studying the erythroid differentiation process and the regulation of gene expression related to erythropoiesis. Their ability to spontaneously differentiate offers a unique advantage for studying the intrinsic pathways and mechanisms that drive erythroid precursor maturation without the addition of external differentiation-inducing agents. Moreover, the differentiation of HEL 92.1.7 cells can be further manipulated through the addition of phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid), which are known to induce macrophage-like differentiation. This induced differentiation into macrophage-like cells expands the utility of the HEL 92.1.7 cell line beyond erythroid studies, allowing researchers to explore and understand the plasticity of hematopoietic cells and the conditions under which lineage commitment and cellular identity can be redirected. Such studies are crucial for developing therapeutic strategies aimed at manipulating cell fate for regenerative medicine and cancer treatment.
Organismo	Human
Tejido	Bone marrow
Enfermedad	Erythroleukemia
Sinónimos	HEL92.1.7, HEL-92.1.7, HEL-92-1-7, HEL-92_1_7, HEL-92, HEL92

Edad	30 years
Género	Male
Origen étnico	Caucasian
Morfología	Round cells
Tipo de célula	Erythroblast
Propiedades de crecimiento	Suspension

Referencia	HEL 92.1.7 (Cytion catalog number 300462)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_2481

Expresión de antígenos	HLA A3, Aw32, Bw35, Ia+
Productos	Hemoglobin, globin (G gamma, A gamma, epsilon, zeta and alpha chains), beta-2-microglobulin, glycophorin

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% heat-inactivated FBS
Reactivo de disociación	Accutase
Subcultivo	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
300462-090425	Certificado de análisis	23. May. 2025	300462
300462-190525	Certificado de análisis	21. Jul. 2025	300462

HEL 92.1.7 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)