HCC1954 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305268

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	The HCC1954 cell line is derived from the primary ductal carcinoma of a human adult breast cancer patient. This cell line is prominently used in breast cancer research, especially for investigating the genetic and molecular characteristics of HER2-positive (HER2+) and triple-negative breast cancers. HCC1954 cells are HER2-overexpressing and possess mutations in the PIK3CA gene, making them a valuable model for studying the signaling pathways involved in cancer progression and the development of targeted therapies. HCC1954 cells exhibit an epithelial morphology and are known for their aggressive growth characteristics both in vitro and in vivo. They express markers associated with aggressive breast cancer phenotypes, including HER2/neu, but lack expression of estrogen receptor (ER) and progesterone receptor (PR), classifying them as triple-negative breast cancer cells. This cell line is extensively used to evaluate the efficacy and mechanisms of action of HER2-targeted therapies, such as trastuzumab, as well as novel PI3K inhibitors. Additionally, HCC1954 cells are employed in research focused on identifying biomarkers for drug resistance and exploring combination treatment strategies to enhance therapeutic outcomes. Their relevance in understanding the biology of aggressive breast cancer and in the development of effective treatments highlights the significance of the HCC1954 cell line in oncological research.
Organismo	Human
Tejido	Breast
Enfermedad	Carcinoma
Sinónimos	HCC-1954, Hamon Cancer Center 1954

Edad	61 years
Género	Female
Origen étnico	East Indian
Morfología	Epithelial
Propiedades de crecimiento	Adherent

Referencia	HCC1954 (Cytion catalog number 305268)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	CVCL_1259

Receptores expresados	Estrogen receptor -, progesteron receptor -
Expresión de proteínas	Epithelial glycoprotein 2 (EGP2), cytokeratin 19
Oncogenes	Her2/neu+ (overexpressed)
Perfil mutacional	Mutation: PIK3CA, p.His1047Arg (c.3140A>G); Mutation: TP53, p.Tyr163Cys (c.488A>G); Gene fusion: CLTC + VMP1 = CLTC-VMP1

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS, add 2.5 g/L glucose, 10 mM HEPES and 1mM sodium pyruvate
Reactivo de disociación	Accutase
Subcultivo	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidad	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305268-280525	Certificado de análisis	21. Jul. 2025	305268
305268-300824	Certificado de análisis	23. May. 2025	305268

HCC1954 Cells

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)