SUM149PT Cells
General information
Organism | Human |
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Tissue | Breast |
Disease | Breast inflammatory carcinoma |
Synonyms | SUM-149PT, SUM 149PT, SUM149-PT, SUM149, SUM-149, SUM 149, 149 PT, 149PT, BrCL12 |
Characteristics
Age | 40 years |
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Gender | Female |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SUM149PT (Cytion catalog number 300609) |
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Expression / Mutation
Protein expression | p53 positive |
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Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 11
TH01: 09. Mrz
TPOX: 9
vWA: 16,18
D3S1358: 17
D21S11: 28,31.2
D18S51: 14,15
Penta E: 11
Penta D: 8,9
D8S1179: 14,16
FGA: 29
D6S1043: 18
D2S1338: 20
D12S391: 15,18
D19S433: 12,14
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