SUM149PT Cells
General information
Description | The SUM149PT cell line is derived from a human inflammatory breast carcinoma (IBC), which represents an aggressive subtype of breast cancer. IBC is characterized by rapid progression, early metastasis, and poor prognosis. SUM149PT cells are classified as triple-negative breast cancer (TNBC), lacking expression of estrogen receptor (ER), progesterone receptor (PR), and HER2 receptor, making them unresponsive to common targeted therapies like endocrine treatments or HER2 inhibitors. Instead, treatment for such cancers typically involves cytotoxic chemotherapy, although these cancers frequently develop resistance over time. Significantly, SUM149PT cells possess a 2288delT BRCA1 mutation, leading to a loss of BRCA1 function. This mutation is a frame-shift deletion that results in premature termination of the BRCA1 protein, impairing DNA repair and promoting genomic instability, a hallmark of BRCA1-mutated cancers. The loss of BRCA1 contributes to the heightened chromosomal instability observed in SUM149PT, which displays numerous chromosomal aberrations. In addition to the mutation, the BRCA1 locus is lost in SUM149PT, further compounding the impact on genomic stability. Surprisingly, SUM149PT cells exhibit a CD44+/CD24-/Low stem-like cancer cell subpopulation, which is enriched in cancer stem cell (CSC) properties such as increased invasion, tumorigenesis, and resistance to chemotherapy. These stem-like cells are also associated with centrosome amplification and elevated cyclin E/Cdk2 activity. Inhibition of Cdk2 in SUM149PT selectively targets this CSC subpopulation, restoring some sensitivity to chemotherapy, which suggests that combined therapeutic strategies targeting Cdk2 and conventional chemotherapy might be effective in treating chemoresistant IBC. |
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Organism | Human |
Tissue | Breast |
Disease | Breast inflammatory carcinoma |
Synonyms | SUM-149PT, SUM 149PT, SUM149-PT, SUM149, SUM-149, SUM 149, 149 PT, 149PT, BrCL12 |
Characteristics
Age | 40 years |
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Gender | Female |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SUM149PT (Cytion catalog number 300609) |
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Expression / Mutation
Protein expression | p53 positive |
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Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 11
TH01: 9.3
TPOX: 9
vWA: 16,18
D3S1358: 17
D21S11: 28,31.2
D18S51: 14,15
Penta E: 11
Penta D: 8,9
D8S1179: 14,16
FGA: 29
D6S1043: 18
D2S1338: 20
D12S391: 15,18
D19S433: 12,14
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