CHO-CTLA4
Required products
Ham's F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
€25.00*
Ham's F-12 Medium, also known as Ham's F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA).
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
44,00
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
122,00
Potassium chloride
223,65
Sodium chloride
7599,00
di-Sodium hydrogen phosphateanhydrous
142,04
Zinc sulfate x 7H2O
0,86
Other Components
D(+)-Glucose anhydrous
1801,60
Hypoxanthine
4,08
Linoleic acid
0,08
DL-α-Lipoic acid
0,21
Phenol red
1,20
Putrescine x 2HCl
0,16
Sodium pyruvate
110,00
Thymidine
0,73
NaHCO3
1176,00
Amino Acids
L-Alanine
8,91
L-Arginine x HCl
210,70
L-Asparagine x H2O
15,01
L-Aspartic acid
13,31
L-Cysteine x HCl x H2O
35,12
L-Alanyl-L-Glutamine
217,30
L-Glutamic acid
14,71
Glycine
7,51
L-Histidine x HCl x H2O
20,96
L-Isoleucine
3,94
L-Leucine
13,12
L-Lysine x HCl
36,54
L-Methionine
4,48
L-Phenylalanine
4,96
L-Proline
34,53
L-Serine
10,51
L-Threonine
11,91
L-Tryptophan
2,04
L-Tyrosine
5,44
L-Valine
11,71
Vitamins
D(+)-Biotin
0,01
D-Calcium pantothenate
0,24
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control
pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product.
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.
Composition
Components
mg/L
Inorganic Salts
Calcium chloride x 2H2O
44,00
Copper(II) sulfate x 5H2O
0,00
Iron (II) sulfate x 7H2O
0,83
Magnesium chloride x 6H2O
122,00
Potassium chloride
223,65
Sodium chloride
7599,00
di-Sodium hydrogen phosphateanhydrous
142,04
Zinc sulfate x 7H2O
0,86
Other Components
D(+)-Glucose anhydrous
1801,60
Hypoxanthine
4,08
Linoleic acid
0,08
DL-α-Lipoic acid
0,21
Phenol red
1,20
Putrescine x 2HCl
0,16
Sodium pyruvate
110,00
Thymidine
0,73
NaHCO3
1176,00
Amino Acids
L-Alanine
8,91
L-Arginine x HCl
210,70
L-Asparagine x H2O
15,01
L-Aspartic acid
13,31
L-Cysteine x HCl x H2O
35,12
L-Alanyl-L-Glutamine
217,30
L-Glutamic acid
14,71
Glycine
7,51
L-Histidine x HCl x H2O
20,96
L-Isoleucine
3,94
L-Leucine
13,12
L-Lysine x HCl
36,54
L-Methionine
4,48
L-Phenylalanine
4,96
L-Proline
34,53
L-Serine
10,51
L-Threonine
11,91
L-Tryptophan
2,04
L-Tyrosine
5,44
L-Valine
11,71
Vitamins
D(+)-Biotin
0,01
D-Calcium pantothenate
0,24
Choline chloride
13,96
Folic acid
1,32
myo-Inositol
18,02
Nicotinamide
0,04
Pyridoxine x HCl
0,06
Riboflavin
0,04
Thiamine x HCl
0,34
Vitamin B12
1,36
DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3
€25.00*
DMEM:Ham's F12 is a widely recognized and extensively utilized basal medium in cell culture for biological research. It serves as a fundamental source of nutrients for the growth of various mammalian cell lines, particularly when supplemented with Fetal Bovine Serum (FBS).
This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.
DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.
To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.
DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +8°C, protected from light.
Once opened, store at 4°C and use within 6–8 weeks.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Amino Acids
Glycine
18.75
L-Alanine
4.45
L-Arginine HCl
147.50
L-Asparagine H₂O
7.50
L-Aspartic Acid
6.65
L-Cysteine HCl H₂O
17.56
L-Cystine 2 HCl
31.29
L-Glutamic Acid
7.35
L-Glutamine
365.00
L-Histidine HCl H₂O
31.48
L-Isoleucine
54.47
L-Leucine
59.05
L-Lysine HCl
91.25
L-Methionine
17.24
L-Phenylalanine
35.48
L-Proline
17.25
L-Serine
26.25
L-Threonine
53.45
L-Tryptophan
9.02
L-Tyrosine Disodium Salt
48.10
L-Valine
52.85
Vitamins
D-Biotin
0.0035
Choline Chloride
8.98
D-Calcium Pantothenate
2.24
Folic Acid
2.66
myo-Inositol
12.60
Nicotinamide
2.02
Pyridoxine HCl
0.031
Pyridoxal HCl
2.00
Riboflavin
0.219
Thiamine HCl
2.17
Vitamin B12
0.68
Inorganic Salts
CaCl₂ 2 H₂O
154.50
CuSO₄ 5 H₂O
0.0013
Fe(NO₃)₃ 9 H₂O
0.05
FeSO₄ 7 H₂O
0.417
KCl
311.80
MgCl₂ 6 H₂O
61.20
MgSO₄
48.84
NaCl
6996.00
NaHCO₃
1200.00
Na₂HPO₄
71.02
NaH₂PO₄
54.30
ZnSO₄ 7 H₂O
0.432
Other Components
D-Glucose
3151.00
Hypoxanthine
2.40
HEPES
3574.50
Linoleic Acid
0.042
Lipoic Acid
0.105
Phenol Red Sodium Salt
8.63
Putrescine 2 HCl
0.081
Sodium Pyruvate
55.00
Thymidine
0.365
This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.
DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.
To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.
DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels.
Quality Control
Sterile-filtered
Storage and Shelf Life
Store at +2°C to +8°C, protected from light.
Once opened, store at 4°C and use within 6–8 weeks.
Shipping Conditions
Ambient temperature
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Avoid freezing and frequent warming to +37°C, as it reduces product quality.
Do not heat the medium beyond 37°C or use uncontrolled heat sources such as microwave appliances.
If only part of the medium is to be used, remove the required amount and warm it to room temperature before use.
Composition
Category
Components
Concentration (mg/L)
Amino Acids
Glycine
18.75
L-Alanine
4.45
L-Arginine HCl
147.50
L-Asparagine H₂O
7.50
L-Aspartic Acid
6.65
L-Cysteine HCl H₂O
17.56
L-Cystine 2 HCl
31.29
L-Glutamic Acid
7.35
L-Glutamine
365.00
L-Histidine HCl H₂O
31.48
L-Isoleucine
54.47
L-Leucine
59.05
L-Lysine HCl
91.25
L-Methionine
17.24
L-Phenylalanine
35.48
L-Proline
17.25
L-Serine
26.25
L-Threonine
53.45
L-Tryptophan
9.02
L-Tyrosine Disodium Salt
48.10
L-Valine
52.85
Vitamins
D-Biotin
0.0035
Choline Chloride
8.98
D-Calcium Pantothenate
2.24
Folic Acid
2.66
myo-Inositol
12.60
Nicotinamide
2.02
Pyridoxine HCl
0.031
Pyridoxal HCl
2.00
Riboflavin
0.219
Thiamine HCl
2.17
Vitamin B12
0.68
Inorganic Salts
CaCl₂ 2 H₂O
154.50
CuSO₄ 5 H₂O
0.0013
Fe(NO₃)₃ 9 H₂O
0.05
FeSO₄ 7 H₂O
0.417
KCl
311.80
MgCl₂ 6 H₂O
61.20
MgSO₄
48.84
NaCl
6996.00
NaHCO₃
1200.00
Na₂HPO₄
71.02
NaH₂PO₄
54.30
ZnSO₄ 7 H₂O
0.432
Other Components
D-Glucose
3151.00
Hypoxanthine
2.40
HEPES
3574.50
Linoleic Acid
0.042
Lipoic Acid
0.105
Phenol Red Sodium Salt
8.63
Putrescine 2 HCl
0.081
Sodium Pyruvate
55.00
Thymidine
0.365