C6 Cells

€430.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 500142

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	The C6 cell line maintains glial cell type with fibroblast morphology and originates from a glioma of a Wisthar-Furth rat. The glioma was induced by exposure to N-nitrosomethylurea, following numerous cycles of alternating culture and animal passages. The C6 glioma cell line is frequently utilized in neuro-oncology research to create animal models that closely mimic the characteristics of human glioma, aiding in the development of new therapeutic agents and strategies. It is particularly effective in 3D cell culture and high-throughput screening. C6 cells are genetically diverse, possessing a wild-type p53 gene, increased Rb gene expression, and a mutant p16/Cdkn2a/Ink4a locus but lacking p16 and p19ARF mRNA expression. They also overexpress several genes in human gliomas, such as PDGFβ, IGF-1, EGFR, and Erb3/Her3 precursor proteins. However, the expression of IGF-2, FGF-9, and FGF-10 is reduced, while MMP-7 gene expression remains unchanged. Like human gliomas, C6 cells show increased activity of the Ras pathway genes, which is regulated by the elevated expression of the Ras guanine triphosphate activator protein. The C6 cell line has been utilized in various studies. For instance, it was used to examine the ability of 2-(2,4-dihydroxy phenyl)thieno-1,3-thiazin-4-one (BChTT) to halt cancer cell proliferation and to investigate the mechanisms involved in this process. In another research, the cytotoxic and antioxidant properties of the supercritical CO2 extract (SCE) of an old man's beard (Usnea barbata) were studied using C6 cells. Interestingly, these cells have been reported to show increased levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids.
Organism	Rat
Tissue	Brain
Disease	Glioma
Synonyms	C-6, C 6, RGC-6, RGC6, RGc6

Age	Unspecified
Gender	Male
Morphology	Fibroblast-like
Cell type	Glial cells
Growth properties	Adherent

Citation	C6 (Cytion catalog number 500142)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_0194

Receptors expressed	Glucocorticoid
Viruses	Positive for LCMV
Virus susceptibility	Vesicular stomatitis (Indiana), vaccinia, herpes simplex
Virus resistance	Poliovirus 3
Reverse transcriptase	Negative
Products	S-100 protein, production of glyceryl phosphate dehydrogenase in response to glucocorticoids, somatotrophin.

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	24 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
500142-34-140624	Certificate of Analysis	23. May. 2025	500142
500142-615-130624	Certificate of Analysis	23. May. 2025	500142

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

C6 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Material Transfer Agreement