B-CPAP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€430.00*

Product number: 305081

Description	B-CPAP is a human papillary thyroid carcinoma cell line that was established from the primary tumor of a 74-year-old woman. The cell line exhibits epithelial-like morphology and is commonly used in research to study thyroid cancer biology, including mechanisms of tumorigenesis and metastasis. B-CPAP cells are notable for harboring a BRAF V600E mutation, which is a common genetic alteration associated with aggressive thyroid cancers and serves as a critical model for evaluating BRAF inhibitors as therapeutic agents. In addition to the BRAF mutation, B-CPAP cells express thyroid-specific markers such as thyroglobulin and thyroid-stimulating hormone receptor, making them a valuable model for studying thyroid gland function and pathology. They have been extensively used in studies investigating the signaling pathways involved in thyroid cancer progression, including MAPK/ERK pathway activation. These cells are also employed in drug resistance and apoptosis studies, providing insights into the mechanisms that might underpin therapeutic failures in thyroid cancer treatments.
Organism	Human
Tissue	Thyroid
Disease	Thyroid carcinoma
Synonyms	BC-PAP, BCPAP

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Doubling time	30 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:5
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,x CSF1PO: 13 D13S317: 12 D16S539: 11,12 D5S818: 10,11 D7S820: 10 TH01: 6,9.3 TPOX: 8,11 vWA: 14,17 D3S1358: 16,17 D21S11: 30,31.2 D18S51: 13,17 Penta E: 5,12 Penta D: 10,11 D8S1179: 12,13 FGA: 20,23 D6S1043: 12,19 D2S1338: 18 D12S391: 18,23 D19S433: 13.2,15

Lot Number	Certificate Type	Date	Catalog Number
305081-070525	Certificate of Analysis	21. Jul. 2025	305081
305081-040923	Certificate of Analysis	23. May. 2025	305081

General information