NCI-N87-Luc 细胞

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

价格不含税，另加运费

cryovial

产品编号 305695

安全数据表

产品介绍

分析证书（CoA）

说明	NCI-N87-Luc is a bioluminescent derivative of the human NCI-N87 gastric adenocarcinoma cell line, engineered to stably express a firefly luciferase reporter gene. The parental NCI-N87 cell line was established from a liver metastasis of a gastric tubular adenocarcinoma in a male patient of African descent and is characterized by HER2 (ERBB2) gene amplification and protein overexpression, making it one of the primary preclinical models for HER2-positive gastric cancer. NCI-N87 cells exhibit epithelial morphology and are widely used to evaluate the efficacy of HER2-targeted therapies including trastuzumab, pertuzumab, and trastuzumab-emtansine (T-DM1). The stable luciferase integration in NCI-N87-Luc enables sensitive, noninvasive bioluminescence imaging (BLI) of tumor burden in subcutaneous and orthotopic xenograft models in immunocompromised hosts. The luminescent signal correlates with viable tumor cell number, supporting longitudinal monitoring of tumor engraftment, growth kinetics, and response to HER2-targeted or chemotherapeutic agents. NCI-N87-Luc is particularly valuable for preclinical evaluation of antibody-drug conjugates (ADCs), bispecific antibodies, and novel HER2-directed strategies in gastric cancer, as well as for high-throughput drug screening applications. NCI-N87-Luc retains the HER2-amplified molecular phenotype and adherent growth characteristics of the parental NCI-N87 line. The luciferase reporter enhances experimental throughput and sensitivity for in vivo imaging studies. Researchers should confirm luciferase activity, HER2 expression status, and growth kinetics under their specific experimental conditions prior to use in quantitative imaging or pharmacological studies.
有机体	Human
组织	Stomach
疾病	Gastric tubular adenocarcinoma
转移部位	Liver
同义词	NCI-N87, NCI N87, N-87, NCI-H87, H87, H-87, NCIN87

年龄	Age unspecified
性别	Male
种族	African
形态学	Epithelial
生长特性	Adherent

引用	NCI-N87-Luc (Cytion catalog number 305695)
生物安全等级	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1603
转基因生物现状	GMO-S1: This cell line contains a stably integrated firefly luciferase reporter cassette (Luc2, codon-optimized) introduced via replication-incompetent lentiviral transduction. The resulting polyclonal cell population was maintained under puromycin selection (1–5 µg/mL). S1 containment is required. This classification applies only within Germany and may differ elsewhere.

抗原表达	Luc2 (firefly, codon-optimized)
致肿瘤性	Yes

培养基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
补充剂	Supplement the medium with 10% FBS, 10 mM HEPES, 2,5g/L Glucose, 1 mM Sodiumpyruvate
解离试剂	Accutase 10 min at 37°C
亚培养	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
分割比率	1 to 3
播种密度	2 to 4 x 10⁴ cells/cm²
流体更新	2 to 3 times per week
冷冻介质	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
解冻和培养细胞	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培养氛围	37°C, 5% CO₂, humidified atmosphere.
运输条件	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
储存条件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

属性 "NCI-N87-Luc 细胞"

转移部位:	肝脏

地段编号	证书类型	日期	目录编号
305695-080526	分析证书	24. Jun. 2026	305695

NCI-N87-Luc 细胞

一般信息

特点

监管数据

生物分子数据

处理

质量控制与分子分析

分析证书（CoA）