Wilms3 Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
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Tissue | Kidney |
Disease | Wilms tumor |
Applications | In vitro cell culture model. Biochemical studies |
Characteristics
Age | 11-12 months |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Spindle-shaped |
Cell type | Wilms cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Wilms3 (Cytion catalog number 300414) |
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Biosafety level | 1 |
Depositor | B. Royer-Pokora |
Expression / Mutation
Mutational profile | WT1 mutation status: homozygous c.1293-1294insA, p.V432fsx87, LOH: 11p11-11pter, CTNNB1 mutation status: wild type |
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Handling
Culture Medium | MSCGM kit (from Lonza) |
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Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 9,11
D5S818: 9,9
D7S820: 10,11
TH01: 6,6
TPOX: 8,8
vWA: 16,17
D3S1358: 15,16
D21S11: 29,31
D18S51: 13,17
Penta E: 7,10
Penta D: 9,13
D8S1179: 10,11
FGA: 22,24
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HLA alleles |
A*: 03:01:01
B*: 35:01:01, 35:03:01
C*: 04:01:01
DRB1*: 04:03:01, 11:04:01
DQA1*: 03:01:01, 05:05:01
DQB1*: 03:01:01, 03:02:01
DPB1*: 01:01:01, 04:01:01
E: 01:03:02, 01:06:01
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