SK-N-LO Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
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Tissue | Brain |
Disease | Primitive Neuroectodermal tumor |
Metastatic site | Bone marrow |
Synonyms | SK-N-L0, SKN-LO, SKNLO |
Characteristics
Age | 10 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent in collagen-coated flasks |
Identifiers / Biosafety / Citation
Citation | SK-N-LO (Cytion catalog number 300400) |
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Biosafety level | 1 |
Expression / Mutation
Karyotype | Phenotype Frequency Product: 0.00005 |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:6 to 1:12 is recommended |
Seeding density | 3 to 4 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 11
TH01: 10
TPOX: 8,11
vWA: 14,17
D3S1358: 14,17
D21S11: 27,28
D18S51: 12
Penta E: 7
Penta D: 9,13
D8S1179: 12,15
FGA: 25
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HLA alleles |
A*: 24:02:01, 29:02:01
B*: 18:01:01, 58:01:01
C*: 05:01:01, 07:18:01
DRB1*: 03:01:01, 08:04:01
DQA1*: 04:01:02, 05:01:01
DQB1*: 02:01:01, 04:02:01
DPB1*: 02:01:02, 13:01:01
E: 01:01, 01:03
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