SK-HEP-1 Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | The SK-HEP-1 cell line is a cancer cell line derived from a liver adenocarcinoma in a 52-year old Caucasian man. It has been shown to form tumors in immunocompromised mice, produce fibronectin, alpha-1 protease inhibitor, and Interleukin-1. However, there is an alternative hypothesis that the cells are of endothelial origin and not hepatocytes. |
---|---|
Organism | Human |
Tissue | Liver |
Disease | Adenocarcinoma |
Metastatic site | Ascites, endothelial cells |
Synonyms | SK-Hep-1, SK HEP-1, SK HEP 01, SK-Hep1, Sk-Hep1, SK Hep1, SKHEP-1, SKHEP1, SKHep1, SK_HEP1 |
Characteristics
Age | 52 years |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-HEP-1 (Cytion catalog number 300334) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Isoenzymes | Me-2, 1-2, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B |
---|---|
Tumorigenic | Yes, in nude mice, forms large cell carcinoma consistent with hepatoma |
Karyotype | (P11) hyperdiploid to hypotriploid (+A3, +C, +E, +F, +G, -A, -D) with abnormalities including dicentrics, acrocentric fragments, secondary constrictions, pulverizations, and large subtelocentric and submetacentric markers |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
TH01: 7,9
TPOX: 9
vWA: 14,17
D3S1358: 16
D21S11: 29,31
D18S51: 13,15
Penta E: 13,21
Penta D: 13,14
D8S1179: 13,14
FGA: 17
D1S1656: 16,17
D6S1043: 11
D2S1338: 20,23
D12S391: 18
D19S433: 12,15.2
|
HLA alleles |
A*: 02:01:01, 24:02:01
B*: 35:02:01, 44:03:01
C*: 04:01:01
DRB1*: 10:01:01, 11:04:01
DQA1*: 01:05:01, 05:05:01
DQB1*: 03:01:01, 05:01:01
DPB1*: 04:01:01
E: 01:01, 01:03
|